Document Detail

Mechanistic studies of the degradation of juvenile hormone esterase in Manduca sexta.
MedLine Citation:
PMID:  9055438     Owner:  NLM     Status:  MEDLINE    
The mechanisms of degradation of juvenile hormone esterase (JHE) were investigated in larvae of the tobacco hornworm, Manduca sexta. JHE is removed from the hemolymph by the pericardial cells by receptor-mediated endocytosis and is ultimately degraded in the lysosomes. Immunoprecipitation experiments and native PAGE followed by Western blotting showed that JHE associates with a putative heat shock cognate protein (Hsp). Approximately 25% of the active JHE in the pericardial cell complex is associated with the putative Hsp 1 h postinjection of affinity purified JHE. Electron microscope analysis revealed that the putative Hsp is located in the trans-Golgi network of pericardial cells, where it is hypothesized to be involved in sorting of proteins destined for the lysosomes, from those destined for the cell membrane. Data acquired from immunoprecipitation and Western blotting experiments argue against the involvement of ubiquitin in the degradation of JHE. Injection of radiolabeled JHE into larvae of M. sexta followed by SDS-PAGE of pericardial cell homogenates revealed covalent binding of an unidentified protein to JHE in the pericardial cell complex.
B C Bonning; T F Booth; B D Hammock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of insect biochemistry and physiology     Volume:  34     ISSN:  0739-4462     ISO Abbreviation:  Arch. Insect Biochem. Physiol.     Publication Date:  1997  
Date Detail:
Created Date:  1997-04-02     Completed Date:  1997-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8501752     Medline TA:  Arch Insect Biochem Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  275-86     Citation Subset:  IM    
Department of Entomology, Iowa State University, Ames, USA.
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MeSH Terms
Carboxylic Ester Hydrolases / isolation & purification,  metabolism*,  ultrastructure
Electrophoresis, Polyacrylamide Gel
Heat-Shock Proteins / isolation & purification,  metabolism,  ultrastructure
Hemolymph / enzymology
Lysosomes / enzymology
Manduca / enzymology*
Microscopy, Electron
Molecular Weight
Recombinant Proteins / metabolism
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Recombinant Proteins; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/juvenile hormone esterase

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