Document Detail

Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions.
MedLine Citation:
PMID:  16387657     Owner:  NLM     Status:  MEDLINE    
The wobble bases of bacterial tRNAs responsible for NNR codons are modified to 5-methylaminomethyl-2-thiouridine (mnm5s2U). 2-thio modification of mnm5s2U is required for accurate decoding and essential for normal cell growth. We identified five genes yhhP, yheL, yheM, yheN, and yccK (named tusA, tusB, tusC, tusD, and tusE, respectively) that are essential for 2-thiouridylation of mnm5s2U by a systematic genome-wide screen ("ribonucleome analysis"). Efficient 2-thiouridine formation in vitro was reconstituted with recombinant TusA, a TusBCD complex, TusE, and previously identified IscS and MnmA. The desulfurase activity of IscS is stimulated by TusA binding. IscS transfers the persulfide sulfur to TusA. TusE binds TusBCD complex and stimulates sulfur transfer from TusA to TusD. TusE also interacts with an MnmA-tRNA complex. This study revealed that 2-thiouridine formation proceeds through a complex sulfur-relay system composed of multiple sulfur mediators that select and facilitate specific sulfur flow to 2-thiouridine from various pathways of sulfur trafficking.
Yoshiho Ikeuchi; Naoki Shigi; Jun-Ichi Kato; Akiko Nishimura; Tsutomu Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular cell     Volume:  21     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-02     Completed Date:  2006-02-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-108     Citation Subset:  IM    
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
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MeSH Terms
Amino Acid Sequence
Carbon-Sulfur Lyases / genetics,  metabolism
Escherichia coli / genetics,  metabolism
Escherichia coli Proteins / genetics,  metabolism
Macromolecular Substances
Molecular Sequence Data
Nucleic Acid Conformation
RNA Processing, Post-Transcriptional
RNA, Bacterial*
RNA, Transfer / chemistry,  genetics,  metabolism*
Recombinant Proteins / genetics,  metabolism
Sequence Alignment
Sulfur / metabolism*
Thiouridine / metabolism*
Reg. No./Substance:
0/Escherichia coli Proteins; 0/Macromolecular Substances; 0/RNA, Bacterial; 0/Recombinant Proteins; 0/tus protein, E coli; 13957-31-8/Thiouridine; 7704-34-9/Sulfur; 9014-25-9/RNA, Transfer; EC 4.4.-/Carbon-Sulfur Lyases; EC 4.4.1.-/cysteine desulfurase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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