Document Detail


Mechanistic basis of otolith formation during teleost inner ear development.
MedLine Citation:
PMID:  21316594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Otoliths, which are connected to stereociliary bundles in the inner ear, serve as inertial sensors for balance. In teleostei, otolith development is critically dependent on flow forces generated by beating cilia; however, the mechanism by which flow controls otolith formation remains unclear. Here, we have developed a noninvasive flow probe using optical tweezers and a viscous flow model in order to demonstrate how the observed hydrodynamics influence otolith assembly. We show that rotational flow stirs and suppresses precursor agglomeration in the core of the cilia-driven vortex. The velocity field correlates with the shape of the otolith and we provide evidence that hydrodynamics is actively involved in controlling otolith morphogenesis. An implication of this hydrodynamic effect is that otolith self-assembly is mediated by the balance between Brownian motion and cilia-driven flow. More generally, this flow feature highlights an alternative biological strategy for controlling particle localization in solution.
Authors:
David Wu; Jonathan B Freund; Scott E Fraser; Julien Vermot
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental cell     Volume:  20     ISSN:  1878-1551     ISO Abbreviation:  Dev. Cell     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-14     Completed Date:  2011-04-01     Revised Date:  2011-09-22    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  271-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Biological Imaging Center, Beckman Institute, Option in Bioengineering, Caltech, Pasadena, CA 91125, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics / physiology
Cilia / physiology
Diffusion
Embryo, Nonmammalian / physiology
Hydrodynamics
Laser Therapy
Movement / physiology
Optical Tweezers
Otolithic Membrane / embryology*,  physiology*
Rheology
Viscosity
Zebrafish / embryology*
Grant Support
ID/Acronym/Agency:
R01 HD043897-04/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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