| Mechanisms underlying impaired GLUT-4 translocation in glycogen-supercompensated muscles of exercised rats. | |
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MedLine Citation:
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PMID: 11093919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise training induces an increase in GLUT-4 in muscle. We previously found that feeding rats a high-carbohydrate diet after exercise, with muscle glycogen supercompensation, results in a decrease in insulin responsiveness so severe that it masks the effect of a training-induced twofold increase in GLUT-4 on insulin-stimulated muscle glucose transport. One purpose of this study was to determine whether insulin signaling is impaired. Maximally insulin-stimulated phosphatidylinositol (PI) 3-kinase activity was not significantly reduced, whereas protein kinase B (PKB) phosphorylation was approximately 50% lower (P < 0.01) in muscles of chow-fed, than in those of fasted, exercise-trained rats. Our second purpose was to determine whether contraction-stimulated glucose transport is also impaired. The stimulation of glucose transport and the increase in cell surface GLUT-4 induced by contractions were both decreased by approximately 65% in glycogen-supercompensated muscles of trained rats. The contraction-stimulated increase in AMP kinase activity, which has been implicated in the activation of glucose transport by contractions, was approximately 80% lower in the muscles of the fed compared with the fasted rats 18 h after exercise. These results show that both the insulin- and contraction-stimulated pathways for muscle glucose transport activation are impaired in glycogen-supercompensated muscles and provide insight regarding possible mechanisms. |
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Authors:
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K Kawanaka; L A Nolte; D H Han; P A Hansen; J O Holloszy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 279 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-06-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1311-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism 3-O-Methylglucose / pharmacokinetics AMP-Activated Protein Kinases Affinity Labels Animals Azides Biological Transport / physiology Dietary Carbohydrates / pharmacology Disaccharides Glucose Transporter Type 4 Glycogen / metabolism* Glycosides Insulin / metabolism Male Membrane Proteins / metabolism Monosaccharide Transport Proteins / metabolism* Multienzyme Complexes / metabolism Muscle Proteins* Muscle, Skeletal / enzymology* Physical Exertion / drug effects, physiology* Propylamines* Protein-Serine-Threonine Kinases / metabolism Rats Rats, Wistar Signal Transduction / physiology Tritium / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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AG-00078/AG/NIA NIH HHS; DK-18968/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Azides; 0/Dietary Carbohydrates; 0/Disaccharides; 0/Glucose Transporter Type 4; 0/Glycosides; 0/Membrane Proteins; 0/Monosaccharide Transport Proteins; 0/Multienzyme Complexes; 0/Muscle Proteins; 0/Propylamines; 0/Slc2a4 protein, rat; 10028-17-8/Tritium; 11061-68-0/Insulin; 129461-18-3/2-N-(4-(1-azitrifluoroethyl)benzoyl)-1,3-bis-(mannos-4-yloxy)-2-propylamine; 146-72-5/3-O-Methylglucose; 9005-79-2/Glycogen; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/AMP-Activated Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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