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Mechanisms that underlie the induction of vasodilation in pulmonary artery by rutin.
MedLine Citation:
PMID:  23222934     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
AIM: Rutin, also known as violaguercitrin, is a bioflavonoid and has a wide range of biological activity. However, the effect of this compound on arteries is not elucidated. Therefore, the objective of this study was to investigate the mechanism underling the induction of vasodilation in pulmonary artery (PA) by the natural product, rutin.
METHODS: Firstly, the isometric tension of the artery rings was studied in vitro with force-electricity transducers. In PA endothelium-intact (EI) rings, rutin elicited concentration-dependent relaxation after the PA rings were pre-contracted by phenylephrine (PE), but induced mesenteric artery (MA) vasoconstriction. Inhibited the endothelial nitric oxide synthase (eNOS) by NG-Nitro-L-arginine Methyl Ester (L-NAME) or removed the PA endothelium would decrease the relaxation effect of rutin.
RESULTS: The NO production was increased in rutin-treated bovine pulmonary artery endothelial cells (BPAECs) detected by fluorescent probe 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM), which verify the functional study results. Moreover, western blot analysis revealed that rutin increased the phosphorylation of eNOS at Ser 1177, but decreased the phosphorylation of eNOS at Thr 495, and did not affect the overall expression of eNOS.
CONCLUSION: These results suggested that rutin-induced relaxation of PAs share NO-eNOS activation pathways, including phosphorylation of Ser 1177, and dephosphorylation at Thr 495. Rutin also has specific action because it exerts a vasodilator influence on the PAs but not MAs.
Authors:
Q Li; S Niu; R Wang; Y Li; R Zhang; D Zhu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International angiology : a journal of the International Union of Angiology     Volume:  31     ISSN:  1827-1839     ISO Abbreviation:  Int Angiol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8402693     Medline TA:  Int Angiol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  557-64     Citation Subset:  IM    
Affiliation:
Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University, Harbin, China.
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