Document Detail


Mechanisms of shock wave induced endothelial cell injury.
MedLine Citation:
PMID:  12355567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Medical procedures, for example, laser angioplasty and extracorporeal lithotripsy as well as high-energy trauma expose human tissues to shock waves (SWs) that may cause tissue injury. The mechanisms for this injury, often affecting blood vessel walls, are poorly understood. Here we sought to assess the role of two suggested factors, viz., cavitation or reactive oxygen species (ROS). STUDY DESIGN/MATERIALS AND METHODS: A laser driven flyer-plate model was used to expose human umbilical cord vein endothelial cell (HUVEC) monolayers to SWs or to SWs plus cavitation (SWC). Cell injury was quantified with morphometry, trypan blue staining, and release of (51)Cr from labeled HUVECs. RESULTS: HUVECs, exposed to SWs only, could not be distinguished from controls in morphological appearance or ability to exclude trypan blue. Yet, release of (51)Cr, indicated a significant cell injury (P < 0.05). HUVEC cultures exposed to SWC, exhibited cell detachment and cell membrane damage detectable with trypan blue. Release of (51)Cr was fourfold compared to SW samples (P < 0.01). Signs of cell injury were evident at 15 minutes and did not change over the next 4 hours. No protective effects of ROS scavengers were demonstrated. CONCLUSIONS: Independent of ROS, SWC generated an immediate cell injury, which can explain, for example, vessel wall perturbation described in relation to SW treatments and trauma.
Authors:
Anders Sondén; Bengt Svensson; Nils Roman; Bo Brismar; Jan Palmblad; B Thomas Kjellström
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  31     ISSN:  0196-8092     ISO Abbreviation:  Lasers Surg Med     Publication Date:  2002  
Date Detail:
Created Date:  2002-09-30     Completed Date:  2003-01-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  233-41     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Affiliation:
Department of Surgery, Söder Hospital, SE-118 83 Stockholm, Sweden. anderssonden@yahoo.se
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MeSH Terms
Descriptor/Qualifier:
Endothelium, Vascular / injuries*,  physiopathology*,  radiation effects
Epithelial Cells / physiology,  radiation effects*
High-Energy Shock Waves / adverse effects*
Humans
Models, Biological
Reactive Oxygen Species / adverse effects*
Trauma Severity Indices
Umbilical Veins / injuries,  physiopathology,  radiation effects
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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