Document Detail


Mechanisms of pulmonary edema induced by a diacylglycerol second messenger.
MedLine Citation:
PMID:  2154134     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effect of dioctanoylglycerol (DOG), a second messenger of protein kinase C (PKC) activation, in the absence and presence of neutrophils in isolated perfused guinea pig lung. DOG was given after a base-line isogravimetric steady-state period. Pulmonary capillary pressure (Ppc) and change in lung weight (delta W) were monitored at 15, 30, and 60 min. Capillary filtration coefficient (Kf,c, an index of vascular permeability) was measured during base-line period and at 30 min. DOG increased the Ppc and delta W at 30 and 60 min, and the Kfc at 30 min. Monooctanoylglycerol, a monoacylglycerol that does not activate PKC, had no effect on Ppc, Kf,c, and delta W. Pretreatment with two different PKC inhibitors, 1-(5-isoquinolinylsulfonyl)-2-methyl piperazine or staurosporin, prevented the pulmonary response to DOG. With neutrophils present, DOG caused greater increases in delta W and the (wet-dry)-to-dry wt ratio compared with DOG group. Response to DOG+ neutrophils was due to oxygen radical production because it was prevented by pretreatment with catalase and because DOG increased superoxide release from neutrophils. PKC activation using DOG in the isolated lung results in pulmonary edema mediated by increases in capillary pressure and vascular permeability. Lung weight-gain response to DOG is greater in the presence of neutrophils. Response to DOG+ neutrophils is mediated by oxygen radicals.
Authors:
A Johnson; D C Hocking; T J Ferro
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  258     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-05     Completed Date:  1990-03-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H85-91     Citation Subset:  IM    
Affiliation:
Research Service, Veterans Administration Medical Center, Albany, New York.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anions / metabolism
Biomechanics
Capillary Permeability / drug effects
Diglycerides* / pharmacology,  physiology
Female
Glycerides* / pharmacology,  physiology
Guinea Pigs
Hemodynamics / drug effects
Lung / anatomy & histology,  drug effects
Male
Neutrophils / metabolism,  physiology
Organ Size / drug effects
Protein Kinase C / antagonists & inhibitors
Pulmonary Circulation / drug effects
Pulmonary Edema / chemically induced*
Second Messenger Systems*
Superoxides / metabolism
Grant Support
ID/Acronym/Agency:
HL-07194-13/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Anions; 0/Diglycerides; 0/Glycerides; 11062-77-4/Superoxides; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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