| Mechanisms of protection afforded by preconditioning to endothelial function against ischemic injury. | |
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MedLine Citation:
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PMID: 8945894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to assess whether the cardioprotective effect of ischemic preconditioning (IPC) on endothelial function in resistance coronary arteries of the rat involves adenosine and/or activation of ATP-sensitive K+ channels (KATP channels). Isolated rat hearts perfused under constant-flow conditions were exposed to 30 min of partial ischemia (flow rate 1 ml/min) followed by 20 min of reperfusion. Preconditioning was performed with 5 min of ischemia and 10 min of reperfusion before the 30-min ischemia. After the 20-min reperfusion period, coronary arteries were precontracted with U-46619 (0.1 microM), and the coronary response to the endothelium-dependent vasodilator serotonin (5-HT; 10 microM) was compared with that of the endothelium-independent vasodilator sodium nitroprusside (SNP; 3 microM). KATP channels or adenosine receptors were blocked with perfusion of either glibenclamide (0.3 microM) or 8-phenyltheophylline (8-PT; 5 microM), respectively, starting 15 min before IPC or a corresponding sham period. In untreated hearts, ischemia selectively diminished 5-HT-induced vasodilation, compared with sham hearts (without ischemia). The vasodilation by SNP was unaffected after ischemia and reperfusion. Preconditioning in untreated hearts preserved the vasodilation produced by 5-HT. Treatment of hearts with either glibenclamide or 8-PT halved the vasodilation produced by both 5-HT and SNP in sham hearts. Glibenclamide reduced by one-half, whereas 8-PT completely blocked, the protective effect of IPC on endothelium-dependent vasodilation. These results suggest that IPC affords protection to endothelial function in resistance coronary arteries of the rat partially by activation of KATP channels. Adenosine plays a major role in that protection. |
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Authors:
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J F Bouchard; D Lamontagne |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1997-01-03 Completed Date: 1997-01-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H1801-6 Citation Subset: IM |
Affiliation:
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Faculté de Pharmacie, Université de Montréal, Quebec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology Animals Coronary Vessels / drug effects, physiopathology Endothelium, Vascular / physiopathology* Glyburide / pharmacology Ischemic Preconditioning, Myocardial* Male Myocardial Ischemia / physiopathology* Potassium Channel Blockers Potassium Channels / drug effects Rats Rats, Sprague-Dawley Receptors, Purinergic P1 / antagonists & inhibitors Theophylline / analogs & derivatives, pharmacology Vascular Resistance / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Potassium Channel Blockers; 0/Potassium Channels; 0/Receptors, Purinergic P1; 10238-21-8/Glyburide; 56-65-5/Adenosine Triphosphate; 58-55-9/Theophylline; 961-45-5/8-phenyltheophylline |
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