| Mechanisms of p,p'-DDE-induced apoptosis in human peripheral blood mononuclear cells. | |
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MedLine Citation:
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PMID: 19545618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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p,p'-Dichlorodiphenyldichloroethylene (DDE), the most stable metabolite of organochlorine insecticide p,p'-dichlorodiphenyltrichloroethane (DDT), has been detected in human populations living in malaria-endemic areas of México where this insecticide was used. DDE induces apoptosis in peripheral blood mononuclear cells (PMBC); however, the molecular mechanism of cell death induced by this compound is poorly understood. In the present study, PBMC isolated from healthy individuals (not exposed to DDE) were incubated in the presence of increasing concentrations of p,p'-DDE (0-80 microg/ml) over time. When PBMC were treated with low p,p'-DDE concentration (10 microg/ml) an antioxidant response and biomarkers of inflammation were induced, indicating a pro-inflammatory state. Moreover, when PBMC were treated with high p,p'-DDE concentration (80 microg/ml) several apoptotic biochemical events were triggered, such as activation of caspase-8, Bid, caspase-9 and caspase-3, as well as degradation of PARP and ubiquitination. The results described in this study show a possible inflammatory condition and the involvement of both extrinsic and intrinsic pathways in the induction of apoptosis in DDE-treated PBMC. |
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Authors:
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Jorge A Alegría-Torres; Fernando Díaz-Barriga; A Jay Gandolfi; Iván N Pérez-Maldonado |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-21 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 23 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-11 Completed Date: 2009-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1000-6 Citation Subset: IM |
Affiliation:
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Departamento de Toxicología Ambiental, Facultad de Medicina, Universidad Autónoma de San Luís Potosí, 78210 San Luís Potosí, Mexico. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apoptosis / drug effects* BH3 Interacting Domain Death Agonist Protein / metabolism Biological Markers / metabolism Caspases / drug effects, metabolism Dichlorodiphenyl Dichloroethylene / administration & dosage, toxicity* Dose-Response Relationship, Drug Humans Inflammation / chemically induced Insecticides / administration & dosage, toxicity* Leukocytes, Mononuclear / drug effects, metabolism Poly(ADP-ribose) Polymerases / drug effects, metabolism Ubiquitination / drug effects Young Adult |
| Chemical | |
Reg. No./Substance:
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0/BH3 Interacting Domain Death Agonist Protein; 0/Biological Markers; 0/Insecticides; 72-55-9/Dichlorodiphenyl Dichloroethylene; EC 2.4.2.30/PARP1 protein, human; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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