| Mechanisms of parenchymal cell death in-vivo after microvascular hemorrhage. | |
| | |
MedLine Citation:
|
PMID: 10708333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: In vitro studies suggest that microhemorrhages with escape of red cells into the tissue may be cytotoxic to parenchymal cells due to oxygen free radical formation. We examined in the rat mesentery the impact of microhemorrhages on parenchymal cell death, as detected by propidium iodide staining, using an intravital approach. METHODS AND RESULTS: Postcapillary venules were punctured with a closed-end micropipette, permitting escape of blood cells and plasma into the mesentery interstitium. Over a period of 2 h, no significant increase in parenchymal cell death was encountered in tissues with hemorrhagic sites compared with nonhemorrhagic control sites. Interstitial microinjections of plasma derived from whole blood incubated for several hours with and without a combination of sodium azide (2 mM) and hydrogen peroxide (1 mM) led to significantly increased levels of cell death compared to control experiments. Interventions against the hydroxyl radical with dimethylthiourea (DMTU, 2 mM) or 2,2'-dipyridyl (DPD, 2 mM), a lipid soluble iron chelator, provided no protective effect against the parenchymal cell death. DMTU slightly delayed tile cytotoxic reaction. CONCLUSIONS: These observations suggest that a newly formed microhemorrhage is not necessarily cytotoxic to parenchymal tissue cells. Interstitial microinjections of plasma, derived from whole blood after prolonged exposure to oxygen free radicals or just aging under in vitro conditions, may be cytotoxic to mesenteric parenchymal cells without effective blockade by interventions against the hydroxyl radical. |
| | |
Authors:
|
H Wilms; F A Delano; G W Schmid-Schönbein |
Related Documents
:
|
17420013 - A quinoxaline 1,4-di-n-oxide derivative induces dna oxidative damage not attenuated by ... 19204183 - Isoforms and functions of nad(p)h oxidase at the macula densa. 14608083 - Choline availability during embryonic development alters progenitor cell mitosis in dev... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Microcirculation (New York, N.Y. : 1994) Volume: 7 ISSN: 1073-9688 ISO Abbreviation: Microcirculation Publication Date: 2000 Feb |
Date Detail:
|
Created Date: 2000-04-11 Completed Date: 2000-04-11 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9434935 Medline TA: Microcirculation Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1-11 Citation Subset: IM |
Affiliation:
|
Department of Bioengineering, Whitaker Institute for Biomedical Engineering, University of California-San Diego, La Jolla 92093-0412, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
2,2'-Dipyridyl
/
pharmacology Animals Cell Death* / drug effects Free Radical Scavengers / pharmacology Free Radicals Hemoglobins / chemistry Hemorrhage / pathology* Hydrogen Peroxide / pharmacology Hydroxyl Radical Iron Chelating Agents / pharmacology Male Mesenteric Veins / injuries Mesentery / blood supply, pathology Microcirculation* Microinjections Oxidative Stress Plasma Rats Rats, Wistar Reactive Oxygen Species Sodium Azide / pharmacology Thiourea / analogs & derivatives, pharmacology Venules / injuries |
| Grant Support | |
ID/Acronym/Agency:
|
HL 43026/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Free Radical Scavengers; 0/Free Radicals; 0/Hemoglobins; 0/Iron Chelating Agents; 0/Reactive Oxygen Species; 26628-22-8/Sodium Azide; 3352-57-6/Hydroxyl Radical; 366-18-7/2,2'-Dipyridyl; 61805-96-7/1,3-dimethylthiourea; 62-56-6/Thiourea; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Variables modulating stress and coping that discriminate drug consumers from low or nondrug consumer...
Next Document: Endothelial cells exposed to anoxia/reoxygenation are hyperadhesive to T-lymphocytes: kinetics and m...