| Mechanisms of glial swelling by arachidonic acid. | |
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MedLine Citation:
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PMID: 7976545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of arachidonic acid (AA, 20:4) was analyzed in vitro by employment of C6 glioma cells and astrocytes from primary culture. The cells were suspended in an incubation chamber under continuous control of pH, pO2, and temperature. Cell swelling was quantified by flow cytometry. After a control period, the suspension was added with AA at concentrations of 0.01 to 1.0 mM. Administration of AA induced an immediate, dose dependent swelling in C6 glioma cells or astrocytes. AA-concentrations of 0.01 mM led to an increase of the glial cell volume to 103.0 +/- 1.0% of control, 0.1 mM to 110.0 +/- 1.5%, and 1.0 mM to 118.8 +/- 1.5% within 10 min. The swelling response to linoleic acid (18:2) was only about half of what was found when AA was administered at a concentration of 0.1 mM, whereas stearic acid (18:0) did not induce any cell volume changes. Inhibition of the cyclo- and lipoxygenase pathway by BW 755C did not prevent glial swelling from AA, whereas it was reduced by SOD, or almost completely abolished by the aminosteroid U-74389F, an antagonist of lipid peroxidation. Replacement of Na(+)- and Cl- -ions in the suspension medium by choline chloride was also associated with complete abolishment of cell swelling from AA. The results demonstrate an impressive efficacy of arachidonic acid to induce glial swelling which might be attributable to activation of lipid peroxidation by the fatty acid, leading to an increased Na(+)-permeability and subsequent influx of water into the cells. |
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Authors:
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F Staub; A Winkler; J Peters; O Kempski; A Baethmann |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta neurochirurgica. Supplementum Volume: 60 ISSN: 0065-1419 ISO Abbreviation: Acta Neurochir Suppl (Wien) Publication Date: 1994 |
Date Detail:
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Created Date: 1994-12-01 Completed Date: 1994-12-01 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0140560 Medline TA: Acta Neurochir Suppl (Wien) Country: AUSTRIA |
Other Details:
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Languages: eng Pagination: 20-3 Citation Subset: IM |
Affiliation:
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Institute for Surgical Research, Ludwig-Maximilians-University, München, Federal Republic of Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / pharmacology* Astrocytes / drug effects Brain Neoplasms Cell Line Cell Membrane Permeability / drug effects Dose-Response Relationship, Drug Flow Cytometry Glioma Intracellular Fluid / drug effects* Linoleic Acid Linoleic Acids / pharmacology Lipid Peroxidation / drug effects Neuroglia / drug effects* Neurons / drug effects Rats Sodium / metabolism Tumor Cells, Cultured / drug effects* Water-Electrolyte Balance / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Linoleic Acids; 2197-37-7/Linoleic Acid; 506-32-1/Arachidonic Acid; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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