Document Detail


Mechanisms of the failure of resting B cells to present tetanus toxoid antigen to T cells.
MedLine Citation:
PMID:  6607279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study examines the failure of resting B cells to present tetanus toxoid (TT) antigen to T cells. A hybrid antibody directed against TT and against human IgM was prepared from immunosorbent-purified rabbit anti-TT and rabbit anti-human IgM. Peripheral blood B cells were prepared by double rosetting with sheep red cells and were depleted of monocytes by double adherence over plastic plates and by passage over a Sephadex G-10 column followed by treatment with OKM1 and complement. B cells were preincubated with medium or with hybrid antibody (60 micrograms/ml) for 1 hr at 4 degrees C, washed, irradiated (2500 R), and tested for their capacity to present TT to an autologous TT-specific T cell clone. B cells coated with the hybrid antibody took up equivalent amounts of 125I-TT as monocytes or EBV-B cells and they internalized fluorescein-labeled TT. In contrast, in the absence of hybrid antibody B cells took up negligible amounts of TT antigen. However, B cells preincubated with the hybrid antibody failed to induce proliferation of autologous TT-specific T cell clones in response to TT. This was not corrected by the addition of partially purified human interleukin I. EBV-B cells sequentially pulsed with hybrid antibody and then with TT for 1 hr at 4 degrees C were equivalent in their capacity to induce T cell proliferation to EBV-B cells presenting soluble TT to the T cells. In contrast EBV-B cells pulsed with TT alone at 4 degrees C failed to take up and present TT. Thus the failure of hybrid antibody-coated resting B cells to present TT reflects an intrinsic inability of the resting B cells to present antigen. Monolayers of hybrid antibody-coated B cells pulsed with TT were prepared on anti-human Fab-coated dishes and were compared with monolayers of TT-pulsed monocytes for their capacity to adsorb TT-specific autologous T cells. In contrast to TT-pulsed monocytes, TT-pulsed hybrid antibody-coated B cells completely failed to bind TT-specific T cells. These results suggest that in addition to their poor antigen uptake resting B cells are incapable of processing antigen and generating the immunogenic moiety recognized by the T cells.
Authors:
C Brozek; D Umetsu; E Schneeberger; R S Geha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  132     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-03-23     Completed Date:  1984-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1144-50     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Anti-Idiotypic / immunology
Antibodies, Bacterial / immunology
Antigens, Bacterial / immunology
B-Lymphocytes / immunology*,  metabolism
Binding Sites, Antibody
Female
Humans
Immunoglobulin M / immunology
Immunosorbents / metabolism
Interleukin-1 / physiology
Lymphocyte Activation
Lymphocyte Cooperation*
Rabbits
T-Lymphocytes / immunology*,  metabolism
Tetanus Toxoid / immunology*
Grant Support
ID/Acronym/Agency:
AI-05877/AI/NIAID NIH HHS; AI-20373/AI/NIAID NIH HHS; AI-K07AI0440-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Binding Sites, Antibody; 0/Immunoglobulin M; 0/Immunosorbents; 0/Interleukin-1; 0/Tetanus Toxoid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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