| Mechanisms controlling the kinetics in proliferation and differentiation of populations of mouse myeloid leukemic cells in vitro. | |
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MedLine Citation:
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PMID: 6943147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The kinetics of differentiation and proliferation of clone cells (B24) of mouse myeloid leukemic M1 cells in vitro were studied by quantitative determination of cellular morphology. B24 cells were induced to differentiate into only macrophagelike cells by an inducer of differentiation in conditioned medium (CM) of embryo cells. During cell differentiation, the ratio of the area of the nucleus to that of the cell (N.C.R.) decreased from about 55% to 10%. Decrease in the N.C.R. was used as an index of cell differentiation in analysis of the kinetics of differentiation of cells treated with various concentrations of CM. The results showed that the process of differentiation was promoted by increasing the concentration of CM, and that the transition of the cells from the undifferentiated state to the differentiated state occurred in a stochastic manner. Comparison of these morphometric results with those of autoradiography showed that the labeling index of the cells decreases gradually in association with decrease in the N.C.R. of the cells from 50% to 30%. A stochastic model for the kinetics of proliferation and differentiation of the cells simulated the experimental observations on the production of differentiated cells. |
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Authors:
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M Hayashi; J Okabe-Kado; M Hozumi |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 108 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1981 Aug |
Date Detail:
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Created Date: 1981-10-25 Completed Date: 1981-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 123-34 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Cell Division Cells, Cultured Culture Media Kinetics Leukemia, Myeloid / pathology* Mice Phagocytosis |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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