Document Detail


Mechanisms of cardiac nerve sprouting after myocardial infarction in dogs.
MedLine Citation:
PMID:  15166093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cardiac nerve sprouting and sympathetic hyperinnervation after myocardial infarction (MI) both contribute to arrhythmogenesis and sudden death. However, the mechanisms responsible for nerve sprouting after MI are unclear. The expression of nerve growth factor (NGF), growth associated protein 43 (GAP43), and other nerve markers were studied at the infarcted site, the noninfarcted left ventricle free wall (LVFW), and the left stellate ganglion (LSG) at several time points (30 minutes to 1 month) after MI. Transcardiac (difference between coronary sinus and aorta) NGF levels were also assayed. Acute MI resulted in the immediate elevation of the transcardiac NGF concentration within 3.5 hours after MI, followed by the upregulation of cardiac NGF and GAP43 expression, which was earlier and more pronounced at the infarcted site than the noninfarcted LVFW. However, cardiac nerve sprouting and sympathetic hyperinnervation were more pronounced in the noninfarcted than the infarcted LVFW site and peaked at 1 week after MI. The NGF and GAP43 protein levels significantly increased in the LSG from 3 days (P<0.01 for all) after MI, without a concomitant increase in mRNA. There was persistent elevation of NGF levels in aorta and coronary sinus within 1 month after MI. We conclude MI results in immediate local NGF release, followed by upregulation of NGF and GAP43 expression at the infarcted site. NGF and GAP43 are transported retrogradely to LSG, which triggers nerve sprouting at the noninfarcted LVFW. A rapid and persistent upregulation of NGF and GAP43 expression at the infarcted site underlies the mechanisms of cardiac nerve sprouting after MI.
Authors:
Shengmei Zhou; Lan S Chen; Yasushi Miyauchi; Mizuho Miyauchi; Saibal Kar; Simon Kangavari; Michael C Fishbein; Behrooz Sharifi; Peng-Sheng Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2004-05-27
Journal Detail:
Title:  Circulation research     Volume:  95     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-09     Completed Date:  2004-11-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  76-83     Citation Subset:  IM    
Affiliation:
Division of Cardiology, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, Calif, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dogs
GAP-43 Protein / metabolism
Gene Expression
Heart / innervation*
Kinetics
Myocardial Infarction / genetics,  metabolism,  physiopathology*
Nerve Growth Factor / metabolism
Nerve Regeneration*
Nerve Tissue Proteins / biosynthesis,  genetics
Stellate Ganglion / metabolism
Sympathetic Nervous System / cytology,  physiology
Grant Support
ID/Acronym/Agency:
HL66389/HL/NHLBI NIH HHS; HL71140/HL/NHLBI NIH HHS; P50 HL52319/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/GAP-43 Protein; 0/Nerve Tissue Proteins; 9061-61-4/Nerve Growth Factor
Comments/Corrections
Comment In:
Circ Res. 2004 Jul 9;95(1):5-6   [PMID:  15242979 ]

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