| Mechanisms of c-reactive protein up-regulation in arterialized vein grafts. | |
| | |
MedLine Citation:
|
PMID: 16455335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: C-reactive protein (CRP), an acute phase reactant, is an independent predictor of coronary artery syndromes and a mediator of the vascular response to injury. CRP has been found in arterialized vein grafts and has been linked to atherogenesis; however, its involvement in vein graft early failure or intimal hyperplasia has not been assessed. This study was designed to investigate the mechanism(s) of CRP up-regulation in arterialized vein grafts. METHODS: Carotid artery bypass with arterialized jugular vein grafts (AVG) was performed in 18 dogs. AVG were harvested at 3, 8, and 24 hours and 4, 14, and 28 days, using the femoral vein obtained at the time of AVG harvest as a control. Serum CRP levels were characterized by enzyme-linked immunosorbent assay; AVG expression of CRP was studied by immunofluorescence, Western blotting, in situ hybridization, Northern blotting, and quantitative RT-PCR. RESULTS: CRP levels peaked at 24 hours in serum and AVG but remained at baseline in control veins. By double immunofluorescence, CRP was associated with the media and adventitia of AVG. However, Northern blotting analysis showed no CRP mRNA expression in AVG. Reverse transcriptase polymerase chain reaction analysis confirmed the lack of up-regulation of CRP in AVG. CONCLUSION: CRP levels are increased in AVG, peaking 24 hours after arterialization. However, no significant production of CRP was detected in AVG. Therefore, increased CRP levels within AVG appear to originate mostly from CRP diffusion from the systemic circulation. These results have significant implications for the development of strategies aimed at blocking CRP up-regulation in bypass grafts. |
| | |
Authors:
|
Iosif Gulkarov; Giuseppe Pintucci; Katja Bohmann; Paul C Saunders; Raymond F Sullivan; Giovanni Ferrari; Paolo Mignatti; Aubrey C Galloway |
Related Documents
:
|
15337895 - The giacomini vein as an autologous conduit in infrainguinal arterial reconstruction. 19496115 - Retrograde recanalization of a chronic total occlusion of a saphenous vein graft. 18692355 - Duplex ultrasound changes in the great saphenous vein after endosaphenous laser occlusi... 11279415 - Chymase-dependent angiotensin ii formation in the saphenous vein versus the internal th... 12615785 - Serum c-reactive protein level is associated with abdominal aortic aneurysm size and ma... 9047065 - Balloon atrial septostomy using a new low-profile balloon catheter: initial clinical re... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Surgery Volume: 139 ISSN: 0039-6060 ISO Abbreviation: Surgery Publication Date: 2006 Feb |
Date Detail:
|
Created Date: 2006-02-03 Completed Date: 2006-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0417347 Medline TA: Surgery Country: United States |
Other Details:
|
Languages: eng Pagination: 254-62 Citation Subset: AIM; IM |
Affiliation:
|
Seymour Cohn Cardiovascular Research Laboratory, Department of Cardiothoracic Surgery, New York, New York, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Atherosclerosis / physiopathology C-Reactive Protein / biosynthesis*, physiology Coronary Artery Bypass* Dogs Enzyme-Linked Immunosorbent Assay Graft Survival Jugular Veins / transplantation* Reverse Transcriptase Polymerase Chain Reaction Up-Regulation |
| Chemical | |
Reg. No./Substance:
|
9007-41-4/C-Reactive Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypoxia/reoxygenation impairs glucose absorption and cAMP-mediated secretion more profoundly than gl...
Next Document: Outcome after hepatic resection versus combined resection and microwave ablation for multiple biloba...