Document Detail

Mechanisms of c-reactive protein up-regulation in arterialized vein grafts.
MedLine Citation:
PMID:  16455335     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: C-reactive protein (CRP), an acute phase reactant, is an independent predictor of coronary artery syndromes and a mediator of the vascular response to injury. CRP has been found in arterialized vein grafts and has been linked to atherogenesis; however, its involvement in vein graft early failure or intimal hyperplasia has not been assessed. This study was designed to investigate the mechanism(s) of CRP up-regulation in arterialized vein grafts. METHODS: Carotid artery bypass with arterialized jugular vein grafts (AVG) was performed in 18 dogs. AVG were harvested at 3, 8, and 24 hours and 4, 14, and 28 days, using the femoral vein obtained at the time of AVG harvest as a control. Serum CRP levels were characterized by enzyme-linked immunosorbent assay; AVG expression of CRP was studied by immunofluorescence, Western blotting, in situ hybridization, Northern blotting, and quantitative RT-PCR. RESULTS: CRP levels peaked at 24 hours in serum and AVG but remained at baseline in control veins. By double immunofluorescence, CRP was associated with the media and adventitia of AVG. However, Northern blotting analysis showed no CRP mRNA expression in AVG. Reverse transcriptase polymerase chain reaction analysis confirmed the lack of up-regulation of CRP in AVG. CONCLUSION: CRP levels are increased in AVG, peaking 24 hours after arterialization. However, no significant production of CRP was detected in AVG. Therefore, increased CRP levels within AVG appear to originate mostly from CRP diffusion from the systemic circulation. These results have significant implications for the development of strategies aimed at blocking CRP up-regulation in bypass grafts.
Iosif Gulkarov; Giuseppe Pintucci; Katja Bohmann; Paul C Saunders; Raymond F Sullivan; Giovanni Ferrari; Paolo Mignatti; Aubrey C Galloway
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Surgery     Volume:  139     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-03     Completed Date:  2006-02-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  United States    
Other Details:
Languages:  eng     Pagination:  254-62     Citation Subset:  AIM; IM    
Seymour Cohn Cardiovascular Research Laboratory, Department of Cardiothoracic Surgery, New York, New York, USA.
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MeSH Terms
Atherosclerosis / physiopathology
C-Reactive Protein / biosynthesis*,  physiology
Coronary Artery Bypass*
Enzyme-Linked Immunosorbent Assay
Graft Survival
Jugular Veins / transplantation*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
9007-41-4/C-Reactive Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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