Document Detail

Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.
MedLine Citation:
PMID:  1532855     Owner:  NLM     Status:  MEDLINE    
The influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.
C M West; J V Moore
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  55     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-05-14     Completed Date:  1992-05-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  425-30     Citation Subset:  IM    
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.
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MeSH Terms
Adenocarcinoma / drug therapy,  metabolism
Antineoplastic Agents / pharmacokinetics,  pharmacology
Biological Transport, Active
Colonic Neoplasms / drug therapy,  metabolism
Drug Resistance
Flow Cytometry
Hematoporphyrin Derivative
Hematoporphyrins / pharmacokinetics,  pharmacology
Radiation-Sensitizing Agents / pharmacokinetics,  pharmacology
Tumor Cells, Cultured / drug effects*,  metabolism
Reg. No./Substance:
0/Antineoplastic Agents; 0/Hematoporphyrins; 0/Radiation-Sensitizing Agents; 68335-15-9/Hematoporphyrin Derivative

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