| Mechanisms of atrial and brain natriuretic peptide release from rat ventricular myocardium: effect of stretching. | |
| | |
MedLine Citation:
|
PMID: 8477647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ventricular hypertrophy is characterized by augmentation of the synthesis and storage of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP). To evaluate in vitro the cellular mechanisms of immunoreactive ANP (IR-ANP) and BNP (IR-BNP) release from ventricular cardiocytes, we measured the secretory response to graded passive myocardial stretch in isolated atrialectomized perfused hypertrophied hearts of 14- to 18-month-old spontaneously hypertensive rats. At this age, the ventricular levels of both IR-ANP and IR-BNP were markedly higher in spontaneously hypertensive (182 +/- 27 and 32 +/- 3 pmol/ventricle, respectively) than in age-matched normotensive Wistar-Kyoto rats (35 +/- 4 and 12 +/- 1 pmol/ventricle, respectively; P < 0.001), whereas the differences between the strains in atrial levels of these peptides were small. The release of natriuretic peptides from ventricles in response to stretch was examined by increasing the volume of the intraventricular balloon for 10 min. Stretching of the hypertrophied ventricles produced a rapid transient (from 1-5 min) increase in both IR-ANP and IR-BNP secretion. As left ventricular pressure rose from 0 to 26 +/- 1 mm Hg, IR-ANP and IR-BNP release into the perfusion fluid increased 1.8 +/- 0.4- and 2.5 +/- 0.2-fold, respectively. Infusion of staurosporine, known to inhibit protein kinase-C activity in heart cells, blocked the stretch-induced increase in IR-ANP release (F = 3.10; P < 0.001, by analysis of variance), but had no effect on basal ventricular IR-ANP secretion (F = 0.87; P = NS). An L-type calcium channel antagonist, diltiazem, had no significant effect on basal (F = 1.20; P = NS) or stretch-stimulated (F = 1.47; P = NS) IR-ANP release from hypertrophied rat myocardium. Chromatographical analysis revealed that the ventricles primarily release the active processed 28- and 45- amino acid ANP- and BNP-like peptides, respectively, both before and during stretch. This study indicates that stretch stimulates both ANP and BNP secretion from hypertropic ventricular myocytes. The results further suggest that protein kinase-C may be involved in stretch-induced ventricular ANP release, whereas the influx of extracellular calcium may not be necessary. |
| | |
Authors:
|
P Kinnunen; O Vuolteenaho; H Ruskoaho |
Related Documents
:
|
7723347 - Defective stretch-induced release of atrial natriuretic peptide from aging hypertensive... 18630737 - Cardiac markers (bnp, nt-pro-bnp, troponin i, troponin t, in female amateur runners bef... 20685687 - Soluble receptor of advanced glycation end products levels are related to ischaemic aet... 18331967 - Bnp and nt-probnp predict echocardiographic severity of diastolic dysfunction. 17599877 - Hybrid coronary artery revascularization--review and update 2007. 1686247 - Effect of congestive heart failure on the intrinsic metabolic capacity of the liver in ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Endocrinology Volume: 132 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1993 May |
Date Detail:
|
Created Date: 1993-05-25 Completed Date: 1993-05-25 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1961-70 Citation Subset: AIM; IM |
Affiliation:
|
Department of Pharmacology and Toxicology, University of Oulu, Finland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkaloids
/
pharmacology Animals Atrial Natriuretic Factor / secretion* Biomechanics Chromatography, High Pressure Liquid Heart / physiopathology* Heart Ventricles / physiopathology Hemodynamics Hypertension / physiopathology Kinetics Male Natriuretic Peptide, Brain Nerve Tissue Proteins / secretion* Rats Rats, Inbred SHR Rats, Inbred WKY Staurosporine |
| Chemical | |
Reg. No./Substance:
|
0/Alkaloids; 0/Nerve Tissue Proteins; 114471-18-0/Natriuretic Peptide, Brain; 62996-74-1/Staurosporine; 85637-73-6/Atrial Natriuretic Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differentiation of ovarian theca-interstitial cells in vitro: regulation of 17 alpha-hydroxylase mes...
Next Document: Enzyme characteristics of two distinct forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-de...