Document Detail


Mechanisms for association of Ca2+/calmodulin-dependent protein kinase II with lipid rafts.
MedLine Citation:
PMID:  16872923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Localization of CaMKIIalpha in lipid rafts was demonstrated in both cultured neurons and mammalian cells transfected with plasmid with an insert of CaMKIIalpha cDNA by using sucrose gradient centrifugation and the sensitivity to a cholesterol-extractor, methyl-beta-cyclodextrin. CaMKIIalpha was targeted to lipid rafts possibly through protein-protein interactions via at least three domains (a.a. 261-309, 371-420, and 421-478). The multimeric structure of the full-length molecule also appeared to contribute to efficient lipid raft-targeting. Acylation of CaMKIIalpha did not appear to be a mechanism for the targeting.
Authors:
Feng Du; Fuminori Saitoh; Qing-Bao Tian; Shoko Miyazawa; Shogo Endo; Tatsuo Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-07
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  347     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-07-28     Completed Date:  2006-09-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  814-20     Citation Subset:  IM    
Affiliation:
Department of Neuroplasticity, Research Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases / genetics,  metabolism*
Cell Fractionation
Cercopithecus aethiops
Hydroxylamine / pharmacology
Membrane Microdomains / drug effects,  enzymology*
Phosphothreonine / metabolism
Protein Binding
Rats
Chemical
Reg. No./Substance:
1114-81-4/Phosphothreonine; 7803-49-8/Hydroxylamine; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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