Document Detail

Mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents.
MedLine Citation:
PMID:  19397979     Owner:  NLM     Status:  MEDLINE    
ETHNOPHARMACOLOGICAL RELEVANCE: Phyllanthus niruri Linn. (Euphorbiaceae) is used as folk medicine in South America to treat excess uric acid. Our initial study showed that the methanol extract of Phyllanthus niruri and its lignans were able to reverse the plasma uric acid of hyperuricemic animals. AIM OF THE STUDY: The study was undertaken to investigate the mechanisms of antihyperuricemic effect of Phyllanthus niruri and its lignan constituents. MATERIAL AND METHODS: The mechanisms were investigated using xanthine oxidase assay and uricosuric studies in potassium oxonate- and uric acid-induced hyperuricemic rats. RESULTS: Phyllanthus niruri methanol extract exhibited in vitro xanthine oxidase inhibition with an IC50 of 39.39 microg/mL and a moderate in vivo xanthine oxidase inhibitory activity. However, the lignans display poor xanthine oxidase inhibition in vitro and a relatively weak in vivo inhibitory activity at 10mg/kg. On the other hand, intraperitoneal treatment with Phyllanthus niruri methanol extract showed 1.69 folds increase in urinary uric acid excretion when compared to the hyperuricemic control animals. Likewise, the lignans, phyllanthin, hypophyllanthin and phyltetralin exhibited up to 2.51 and 11.0 folds higher in urinary uric acid excretion and clearance, respectively. The co-administration of pyrazinamide with phyllanthin exhibited a significant suppression of phyllanthin's uricosuric activity resembling that of pyrazinamide with benzbromarone. CONCLUSIONS: The present study showed that the antihyperuricemic effect of Phyllanthus niruri methanol extract may be mainly due to its uricosuric action and partly through xanthine oxidase inhibition, whereas the antihyperuricemic effect of the lignans was attributed to their uricosuric action.
Vikneswaran Murugaiyah; Kit-Lam Chan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-03
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  124     ISSN:  1872-7573     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-29     Completed Date:  2009-10-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  233-9     Citation Subset:  IM    
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
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MeSH Terms
Disease Models, Animal
Drug Synergism
Gout Suppressants / isolation & purification,  pharmacology,  therapeutic use*
Hyperuricemia / chemically induced,  drug therapy,  urine
Inhibitory Concentration 50
Lignans / isolation & purification,  pharmacology,  therapeutic use*
Molecular Structure
Phyllanthus / chemistry*
Plant Extracts / chemistry,  pharmacology,  therapeutic use*
Plant Leaves
Pyrazinamide / pharmacology,  therapeutic use
Rats, Sprague-Dawley
Uric Acid / urine*
Urine / chemistry
Xanthine Oxidase / antagonists & inhibitors*
Reg. No./Substance:
0/Gout Suppressants; 0/Lignans; 0/Plant Extracts; 69-93-2/Uric Acid; 98-96-4/Pyrazinamide; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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