| Mechanisms of the adjuvant effect of hemoglobin in experimental peritonitis. VII. Hemoglobin does not inhibit clearance of Escherichia coli from the peritoneal cavity. | |
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MedLine Citation:
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PMID: 6351314 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hemoglobin has been shown to be a potent adjuvant in experimental Escherichia coli peritonitis, although a satisfactory mechanistic rationale is still obscure. Hemoglobin has been thought to impair intraperitoneal neutrophil function, delay clearance of bacteria from the peritoneal cavity by the normal absorptive mechanisms, or directly enhance bacterial growth. Using highly purified stroma-free hemoglobin (SFHgb), we have largely discounted any direct effect of hemoglobin on peritoneal white blood cell function. In the present study, we confirmed that uncontrolled proliferation of bacteria takes place in the presence of hemoglobin in the peritoneal cavity. Nonviable 5-iododeoxyuridine 125I-labelled bacteria were then used to directly study peritoneal clearance kinetics, eliminating the problem of bacterial growth. SFHgb had no influence on the removal of intraperitoneal bacteria. The rate of bloodstream appearance of radiolabel was similar with or without intraperitoneal SFHgb. Thus, SFHgb does not prevent clearance of bacteria from the peritoneal cavity by interfering with normal host clearance mechanisms. SFHgb may act as a bacterial growth adjuvant, either by serving as a bacterial nutrient or by suitably modifying the environment so that extensive bacterial proliferation can occur. The latter hypothesis appears to be an area in which investigation concerning the adjuvant effect of hemoglobin may prove most fruitful. |
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Authors:
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D L Dunn; R A Barke; J T Lee; R M Condie; E W Humphrey; R L Simmons |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Surgery Volume: 94 ISSN: 0039-6060 ISO Abbreviation: Surgery Publication Date: 1983 Sep |
Date Detail:
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Created Date: 1983-10-08 Completed Date: 1983-10-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0417347 Medline TA: Surgery Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 487-93 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Escherichia coli / growth & development, physiology* Escherichia coli Infections / microbiology* Hemoglobins / physiology* Humans Idoxuridine / diagnostic use Iodine Radioisotopes / diagnostic use Kinetics Male Peritoneal Cavity / microbiology* Peritonitis / microbiology* Rats Rats, Inbred Strains Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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AI 14032/AI/NIAID NIH HHS; AM 0635301/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 0/Iodine Radioisotopes; 54-42-2/Idoxuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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