Document Detail


Mechanisms of acid secretion in pseudobranch cells of rainbow trout (Oncorhynchus mykiss).
MedLine Citation:
PMID:  12177159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell suspensions of rainbow trout Oncorhynchus mykiss pseudobranch, prepared by Ca(2+) depletion and mechanical maceration, contained a distinct population of cells that always kept their relatively cuboidal shape and did not round up in suspension or proliferate after adhering to the surface of cell culture dishes. Phasecontrast microscopy revealed an extensive system of basal membrane invaginations, and Na(+)-K(+)-ATPase- and anionexchanger-like immunoreactivity could be localized in cell membranes. The cells were characterized by a high mitochondrial density. Using specific antibodies, V-ATPase subunit B was localized in the plasma membrane. Using a cytosensor microphysiometer, the rate of acid secretion of these cells was measured and compared with the activity of a gill cell preparation. Incubation of pseudobranch cells with bafilomycin A1 (10(-6) mol l(-1)), a specific inhibitor of V-ATPase, reduced the rate of acid secretion by about 10% under control conditions, while no effect of bafilomycin on the rate of acid secretion of gill cells was observed. Application of amiloride (5 x 10(-5) mol l(-1)) reduced the rate of acid secretion in cells of both organs, pseudobranch and gills. Incubation of pseudobranch cells with DIDS (10(-3) mol l(-1)) resulted in a minor increase in the rate of proton secretion, but in cells prepared from the gills of rainbow trout acid secretion was reduced by about 30-40%. It is concluded that pseudobranch cells are equipped with various pathways to secrete protons, and that the anion exchange activity especially of pseudobranch cells appears to be different from that in gills.
Authors:
G Kern; S T Bösch; E Unterhuber; B Pelster
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  205     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-14     Completed Date:  2003-03-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2943-54     Citation Subset:  IM    
Affiliation:
Institut für Zoologie und Limnologie, Universität Innsbruck, Technikerstrasse 25, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology
Cell Membrane / enzymology
Cell Physiological Phenomena
Cells / cytology,  drug effects
Cells, Cultured
Gills / physiology
Hydrogen-Ion Concentration*
Immunohistochemistry
Keratins / analysis
Macrolides*
Mitochondria / metabolism
Oncorhynchus mykiss / physiology*
Protein Subunits / analysis
Sodium-Potassium-Exchanging ATPase / metabolism*
Vacuolar Proton-Translocating ATPases / metabolism*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Macrolides; 0/Protein Subunits; 68238-35-7/Keratins; 88899-55-2/bafilomycin A1; EC 3.6.1.-/Vacuolar Proton-Translocating ATPases; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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