Document Detail


Mechanisms regulating monocyte CXCL8 secretion in neurocysticercosis and the effect of antiparasitic therapy.
MedLine Citation:
PMID:  20826750     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurocysticercosis (NCC) due to infection with Taenia solium is a major cause of epilepsy worldwide. Larval degeneration, which may follow antiparasitic treatment, results in clinical symptoms due to inflammatory cell influx. Mechanisms regulating this are not well understood, but chemokines have a key role. Stimulation of human monocytes by cyst Ags from NCC-infected pigs showed that scolex and membrane Ags drive CXCL8 and CCL2 secretion. Antiparasitic treatment of pigs increased CXCL8 in response to brain, but not muscle, cyst Ags. Cyst-fluid Ags did not elicit monocyte chemokine secretion, inhibited LPS-induced CXCL8 by up to 89%, but did not alter CCL2 secretion. This effect was inhibited by anti-IL-10 Abs. Plasma CXCL8, TNF-α, IL-10, eotaxin, IL-1, IL-1ra, soluble IL-1R-II, and soluble TNFR-I and -II levels were evaluated in 167 NCC patients. Patients had lower plasma CXCL8 and TNF-α concentrations than control subjects. In summary, larval Ags from brain and muscle cysts differentially regulate chemokine secretion. Cyst-fluid inhibits CXCL8, and this is blocked by anti-IL-10 Abs. CXCL8 concentrations are decreased in patient plasma. Following anti-parasitic therapy, scolex and membrane Ags are exposed, and cyst fluid is decreased, leading to inflammatory cell influx. Taken together, the cellular, porcine, and human data may explain, in part, why NCC is usually asymptomatic but may cause proinflammatory symptoms, particularly following treatment.
Authors:
Jasim Uddin; Armando E Gonzalez; Robert H Gilman; Lynette H Thomas; Silvia Rodriguez; Carlton A W Evans; Daniel G Remick; Hector H Garcia; Jon S Friedland
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-08
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  185     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2010-10-19     Revised Date:  2011-07-25    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4478-84     Citation Subset:  AIM; IM    
Affiliation:
Department of Infectious Diseases and Immunity, Wellcome Trust Centre for Clinical Tropical Medicine, Imperial College London, London, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Helminth
Antiparasitic Agents / pharmacology*
Cells, Cultured
Chemokines / immunology,  metabolism
Cysts / immunology
Humans
Inflammation / immunology,  metabolism
Interleukin-8 / drug effects,  immunology,  metabolism*
Monocytes / immunology,  metabolism*
Neurocysticercosis / drug therapy*,  immunology,  metabolism*
Swine
Grant Support
ID/Acronym/Agency:
057434//Wellcome Trust; AI-35894/AI/NIAID NIH HHS; AI-42037-01/AI/NIAID NIH HHS; TW001140/TW/FIC NIH HHS; TW00910/TW/FIC NIH HHS; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Antigens, Helminth; 0/Antiparasitic Agents; 0/Chemokines; 0/Interleukin-8
Comments/Corrections

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