Document Detail

Mechanism of visible light phototoxicity on Porphyromonas gingivalis and Fusobacterium nucleatum.
MedLine Citation:
PMID:  15960594     Owner:  NLM     Status:  MEDLINE    
Phototoxicity of visible light laser on the porphyrin-producing bacteria, Porphyromonas gingivalis, in the absence of photosensitizers and under aerobic conditions was shown in previous studies. Recently, we found that the noncoherent visible light sources at wavelengths of 400-500 nm, commonly used in restorative dentistry, induced a phototoxic effect on P. gingivalis, as well as on Fusobacterium nucleatum, and to a lesser extent on the Streptococci sp. To elucidate the mechanism of this phototoxic effect, P. gingivalis and F. nucleatum were exposed to light (1) under aerobic and anaerobic environments and (2) in the presence of scavengers of reactive oxygen species (ROS). Phototoxic effect was not observed when the bacteria were exposed to light under anaerobic conditions. Dimethyl thiourea, a hydroxyl radical scavenger, was effective in reducing phototoxicity (P </= 0.05). Other scavengers, such as catalase, superoxide dismutase and ascorbic acid, were less effective when applied separately. These results support the assumption that the phototoxic effect of blue light on the periopathogenic bacteria is oxygen dependent and that hydroxyl radicals play an important role in this process.
Osnat Feuerstein; Isaac Ginsburg; Eti Dayan; Dalya Veler; Ervin I Weiss
Related Documents :
15096614 - Bistable uv pigment in the lamprey pineal.
19930124 - Determinants of pathology in light-irradiated cells.
20356294 - Using light-induced thermocleavage in a roll-to-roll process for polymer solar cells.
20060494 - Influence of the dark/light rhythm on the effects of uv radiation in the eyestalk of th...
12117544 - Alterations of basilar membrane response phase and velocity after acoustic overstimulat...
21410494 - Early biting rhythm in the afro-tropical vector of malaria, anopheles arabiensis, and c...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  81     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:    2005 Sep-Oct
Date Detail:
Created Date:  2005-11-04     Completed Date:  2006-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1186-9     Citation Subset:  IM    
Department of Prosthodontics, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Fusobacterium nucleatum / radiation effects*
Microbial Viability
Porphyromonas gingivalis / radiation effects*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Enzyme-assisted reforming of glucose to hydrogen in a photoelectrochemical cell.
Next Document:  Fluorescence spectroscopic study of hypericin-photosensitized oxidation of low-density lipoproteins.