Document Detail

Mechanism of sucrose uptake by isolated rat hepatocytes.
MedLine Citation:
PMID:  3667704     Owner:  NLM     Status:  MEDLINE    
The transport of molecules by nonspecific endocytosis has been described in many cell types, but it has not been characterized in hepatocytes. Because of its central role in the clearance of solutes from portal blood, endocytosis might represent a significant mode of cellular transport. We investigated the mechanism of sucrose uptake in an isolated hepatocyte system. Liver cells were isolated by perfusion and collagenization of rat liver, followed by differential centrifugation. Hepatocytes were then incubated with 14C-sucrose and harvested by spinning through oil in microfuge tubes. Radioactivity was standardized against DNA content. We found that sucrose uptake is concentration-dependent from 5 microM to 100 mM and follows first-order kinetics. Washout studies indicate that exocytosis is responsible for the dynamic equilibrium reached. Arrhenius analysis of temperature dependence yields a linear plot (Ea = 14.2 Kcal/mol). In addition, sucrose uptake is independent of cellular ATP levels. We conclude that sucrose is transported by fluid-phase micropinocytosis in isolated hepatocytes and that this transport mechanism may be important in the uptake of diverse molecules into liver cells.
A W Sasaki; S K Williams; M Jain; R C Wagner
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  133     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1987-12-17     Completed Date:  1987-12-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  175-80     Citation Subset:  IM    
School of Life and Health Sciences, University of Delaware, Newark 19711.
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MeSH Terms
Adenosine Triphosphate / pharmacology
Biological Transport
Dose-Response Relationship, Drug
Liver / metabolism*
Rats, Inbred Strains
Sucrose / metabolism*
Grant Support
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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