| Mechanism of sucrose uptake by isolated rat hepatocytes. | |
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MedLine Citation:
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PMID: 3667704 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The transport of molecules by nonspecific endocytosis has been described in many cell types, but it has not been characterized in hepatocytes. Because of its central role in the clearance of solutes from portal blood, endocytosis might represent a significant mode of cellular transport. We investigated the mechanism of sucrose uptake in an isolated hepatocyte system. Liver cells were isolated by perfusion and collagenization of rat liver, followed by differential centrifugation. Hepatocytes were then incubated with 14C-sucrose and harvested by spinning through oil in microfuge tubes. Radioactivity was standardized against DNA content. We found that sucrose uptake is concentration-dependent from 5 microM to 100 mM and follows first-order kinetics. Washout studies indicate that exocytosis is responsible for the dynamic equilibrium reached. Arrhenius analysis of temperature dependence yields a linear plot (Ea = 14.2 Kcal/mol). In addition, sucrose uptake is independent of cellular ATP levels. We conclude that sucrose is transported by fluid-phase micropinocytosis in isolated hepatocytes and that this transport mechanism may be important in the uptake of diverse molecules into liver cells. |
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Authors:
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A W Sasaki; S K Williams; M Jain; R C Wagner |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 133 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1987 Oct |
Date Detail:
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Created Date: 1987-12-17 Completed Date: 1987-12-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 175-80 Citation Subset: IM |
Affiliation:
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School of Life and Health Sciences, University of Delaware, Newark 19711. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology Animals Biological Transport Dose-Response Relationship, Drug Glycolysis Liver / metabolism* Male Pinocytosis* Rats Rats, Inbred Strains Sucrose / metabolism* Temperature |
| Grant Support | |
ID/Acronym/Agency:
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HL16666/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate; 57-50-1/Sucrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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