Document Detail


Mechanism and specificity of reconstitution of dimeric lactate dehydrogenase from Limulus polyphemus.
MedLine Citation:
PMID:  4005047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D-Lactate dehydrogenase (EC 1.1.1.28) from Limulus polyphemus is a homodimer which is composed of identical subunits of Mr = 35 000. The enzyme may be reversibly denatured and dissociated at acid pH or in 6M guanidine X HCl. The sigmoidal time course of reactivation obeys a consecutive uni-bimolecular mechanism with k1 = 6 X 10(-4) S-1 and k2 = 1.3 X 10(-4) M-1 S-1 (20 degrees C) as first- and second-order rate constants. Cross-linking experiments with glutaraldehyde prove that reactivation and dimer formation run parallel. Joint "synchronous" reconstitution of the enzyme with dimeric porcine mitochondrial malate dehydrogenase (after denaturation in 6M guanidine X HCl) does not yield active hybrids. The unchanged kinetics of reactivation in the absence and presence of the prospective partner of hybridization prove that inactive hybrid intermediates may also be excluded. The absence of hybrids upon synchronous reconstitution of the two closely related dimeric NAD-dependent dehydrogenases clearly suggests that the assembly of nascent oligomeric proteins must be highly specific.
Authors:
M Gerl; R Rudolph; R Jaenicke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry Hoppe-Seyler     Volume:  366     ISSN:  0177-3593     ISO Abbreviation:  Biol. Chem. Hoppe-Seyler     Publication Date:  1985 May 
Date Detail:
Created Date:  1985-08-22     Completed Date:  1985-08-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503054     Medline TA:  Biol Chem Hoppe Seyler     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  447-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Guanidine
Guanidines / pharmacology
Horseshoe Crabs / enzymology*
Hydrogen-Ion Concentration
Kinetics
L-Lactate Dehydrogenase / isolation & purification,  metabolism*
Macromolecular Substances
Molecular Weight
Protein Denaturation
Chemical
Reg. No./Substance:
0/Guanidines; 0/Macromolecular Substances; 113-00-8/Guanidine; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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