Document Detail

Mechanism of replication of bacteriophage phi X174 XIX. Initiation of phi X174 viral strand DNA synthesis at internal sites on the genome.
MedLine Citation:
PMID:  6211552     Owner:  NLM     Status:  MEDLINE    
Bacteriophage phi X174 viral strand DNA molecules shorter than genome length found late in the infectious cycle in Escherichia coli were 5' end labeled with 32P. Hybridization of the 32P-labeled molecules to restriction enzyme fragments of phi X replicative form DNA revealed an excess of phi X molecules whose 5' ends mapped in HaeIII fragments Z3 and Z4 in comparison with fragments Z1 and Z2. This suggests that initiation of phi X174 viral strand DNA synthesis may occur at internal sites on the complementary strand. There are several appropriately located sequences that might serve as n' (factor Y) recognition sequences and thereby facilitate discontinuous synthesis of the viral strand.
M Matthes; P J Weisbeek; D T Denhardt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  42     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-08-14     Completed Date:  1982-08-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-5     Citation Subset:  IM    
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MeSH Terms
Bacteriophage phi X 174 / genetics*
Base Sequence
DNA Replication*
DNA, Viral / biosynthesis*
Models, Genetic
Nucleic Acid Hybridization
Virus Replication*
Reg. No./Substance:
0/DNA, Viral

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