Document Detail


Mechanism of pppA(2'p5'A)n2'p5'AOH synthesis in extracts of interferon-treated HeLa cells.
MedLine Citation:
PMID:  6154699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment of HeLa cells with interferon results in the induction of an enzymatic activity designated 2'5'oligo(A) polymerase. The polymerase requires continuous presence of double-stranded RNA (dsRNA) for activity, since degradation of dsRNA abolishes synthesis of the oligomeric series pppA(2'p5'A)n. These oligonucleotides are formed initially at a constant rate with dimer synthesized faster than trimer, and the latter faster than tetramer. After 45 min, accumulation of the dimer declines whereas that of other oligomers still proceeds at a linear rate. These results suggest that an oligomer remains associated with the enzyme for possible consecutive additions of adenylate, since no significant accumulation of dimer precedes synthesis of trimer. The relative amounts of the different oligomers found at the end of a reaction may reflect an increasing probability of release as the oligomers are elongated. The accumulation of dimer, however, decreases when it becomes a substrate for adenylate addition; incorporation of isolated dimer into 2'5'-oligo(A) was directly shown. Other nucleotides with a blocked p5'A terminus, like A5'ppppp5'A and NADH, can serve as adenylate acceptors in the presence of dsRNA. The adenosine triphosphates 2'-dATP and 3'-dATP are not incorporated efficiently into 2'5'-oligo(A) and inhibit its synthesis.
Authors:
M A Minks; S Benvin; C Baglioni
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  255     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1980 Jun 
Date Detail:
Created Date:  1980-07-26     Completed Date:  1980-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5031-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2',5'-Oligoadenylate Synthetase
Adenine Nucleotides / biosynthesis*
Enzyme Induction
Hela Cells / drug effects,  enzymology
Humans
Interferons / pharmacology*
Kinetics
Nucleotides / pharmacology
Oligonucleotides / biosynthesis*
Oligoribonucleotides / biosynthesis*
Polynucleotide Ligases / biosynthesis*
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 0/Nucleotides; 0/Oligonucleotides; 0/Oligoribonucleotides; 0/adenosine triphosphate-2',5'-adenosine monophosphate; 9008-11-1/Interferons; EC 2.7.7.-/2',5'-Oligoadenylate Synthetase; EC 6.5.1.-/Polynucleotide Ligases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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