Document Detail

Mechanism of porphyrin-induced sonodynamic effect: possible role of hyperthermia.
MedLine Citation:
PMID:  16494518     Owner:  NLM     Status:  MEDLINE    
The biological effects of ultrasound have been investigated vigorously for various applications including the thermal coagulation of tissues, the opening of tight junctions, and localized gene or drug introduction. The synergistic cell killing effect of ultrasound and porphyrin derivatives, the so-called sonodynamic effect, holds promise for cancer treatment. Although several models to explain the sonodynamic effect have been proposed, its exact mechanism, especially in vivo, remains unknown. We examined the effect of a porphyrin derivative, protoporphyrin IX, on ultrasound-induced killing of HeLa cells. In some experiments, the intracellular protoporphyrin IX concentration was increased by 5-aminolevulinic acid treatment of the cells. Although extracellular protoporphyrin IX showed an enhanced cell killing effect by microbubble-enhanced ultrasound, intracellular protoporphyrin IX did not. On the other hand, intracellular protoporphyrin IX enhanced the cell killing effect of hyperthermia, which can be produced by ultrasound exposure, in a moderately acidic environment (pH 6.6). Because porphyrin derivatives are generally imported into the intracellular component in vivo, our results suggest that hyperthermia caused by ultrasound may play an important role in the sonodynamic effect induced by porphyrin derivatives.
Manabu Kinoshita; Kullervo Hynynen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  165     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-23     Completed Date:  2006-04-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  299-306     Citation Subset:  IM; S    
Department of Radiology, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA.
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MeSH Terms
Aminolevulinic Acid / pharmacology
Cell Survival / drug effects
Fever / pathology*
Hela Cells
Hydrogen-Ion Concentration
Porphyrins / pharmacology*
Grant Support
Reg. No./Substance:
0/Porphyrins; 106-60-5/Aminolevulinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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