| Mechanism of the incidental production of a melanin-like pigment during 6-demethylchlortetracycline production in Streptomyces aureofaciens. | |
| | |
MedLine Citation:
|
PMID: 10742218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The secondary metabolite 6-demethylchlortetracycline (6-DCT), which is produced by Streptomyces aureofaciens, is used as a precursor of semisynthetic tetracyclines. Strains that produce 6-DCT also produce a melanin-like pigment (MP). The correlation between MP production and 6-DCT production was investigated by using S. aureofaciens NRRL 3203. Production of both MP and 6-DCT was repressed by phosphate or ammonium ions, suggesting that syntheses of these compounds are controlled by the same regulators. Ten chlortetracycline-producing recombinants were derived from 6-DCT-producing mutant NRRL 3203 by gene replacement. All of the recombinants produced chlortetracycline but not MP, indicating that MP production is the results of a defect in the 6-methylation step and suggesting that the polyketide nonaketideamide is a common intermediate leading to MP as well as 6-DCT. To further examine the possibility that MP might be synthesized via the 6-DCT-biosynthetic pathway, mutants defective in 6-DCT biosynthesis were derived from a 6-DCT producer. Some of these mutants were able to produce MP, while others, including mutants with mutations in the gene encoding anhydrotetracycline oxygenase, an enzyme catalyzing the penultimate step in the pathway, produced neither 6-DCT nor MP. Production of 6-DCT and production of MP were restored simultaneously by integrative transformation with the corresponding 6-DCT-biosynthetic genes, indicating that some of 6-DCT-biosynthetic enzymes are indispensable for MP production. These findings suggest that a defect in the 6-methylation step results in redirection of carbon flux from a certain intermediate in the 6-DCT-biosynthetic pathway to a shunt pathway and results in MP production. |
| | |
Authors:
|
T Nakano; K Miyake; M Ikeda; T Mizukami; R Katsumata |
Related Documents
:
|
18957398 - Emergence and widespread dissemination of oxa-23, -24/40 and -58 carbapenemases among a... 10906148 - Cyclization of polyubiquitin by the e2-25k ubiquitin conjugating enzyme. 11284028 - Is (9z)-"meso"-zeaxanthin optically active? 18489708 - Hinokinin biosynthesis in linum corymbulosum reichenb. 14710118 - The history of cesarean technique. 17892088 - Retelling urban legends. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 66 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2000 Apr |
Date Detail:
|
Created Date: 2000-05-11 Completed Date: 2000-05-11 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1400-4 Citation Subset: IM |
Affiliation:
|
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida, Tokyo 194-8533, Japan. t.nakano@kyowa.co.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
biosynthesis* Culture Media Demeclocycline / biosynthesis* Gene Expression Regulation, Bacterial Melanins / biosynthesis* Methylation Multigene Family Mutation Pigments, Biological / biosynthesis* Plasmids / genetics Recombination, Genetic Streptomyces aureofaciens / genetics, growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Culture Media; 0/Melanins; 0/Pigments, Biological; 127-33-3/Demeclocycline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glutathione-dependent conversion of N-ethylmaleimide to the maleamic acid by Escherichia coli: an in...
Next Document: Influence of catalase and superoxide dismutase on ozone inactivation of Listeria monocytogenes.