Document Detail


Mechanism of hydrolysis of a novel indolocarbazole topoisomerase I inhibitor.
MedLine Citation:
PMID:  20025967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The degradation kinetics and reaction product profile of the antitumor agent 1 in aqueous solution was studied. Hydrolysis of the pendant imide ring of 1 is the primary mode of thermal degradation in aqueous solution, and the pH rate profile of 1 has a V-shape indicating that hydrolysis of the imide ring can be catalyzed by either acid or base. Hydrolysis of 1 to the anhydride derivative 3 or the dicarboxylic acid derivative 4 is stepwise and the intermediates 2a and 2b formed by initial hydrolytic attack have been observed under alkaline conditions. An overall mechanism for the hydrolysis of 1 in aqueous solution has been proposed. Extrapolating Arrhenius behavior to the hydrolysis reaction of 1 in aqueous solution maintained at a pH value of 4 suggests an aqueous buffered formulation has sufficient thermal stability to be considered a robust room temperature drug product.
Authors:
David Breslin; Yuichi Sato; Shyam B Karki
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Publication Detail:
Type:  Journal Article     Date:  2009-12-16
Journal Detail:
Title:  European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences     Volume:  39     ISSN:  1879-0720     ISO Abbreviation:  Eur J Pharm Sci     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-07-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9317982     Medline TA:  Eur J Pharm Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  291-7     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
Pharmaceutical Research and Development, Merck Research Laboratories, West Point, PA 19486, USA.
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MeSH Terms
Descriptor/Qualifier:
Carbazoles / chemistry,  pharmacology*
DNA Topoisomerases, Type I / chemistry,  pharmacology*
Electrophoresis, Capillary
Enzyme Inhibitors / chemistry,  pharmacology*
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Chemical
Reg. No./Substance:
0/Carbazoles; 0/Enzyme Inhibitors; EC 5.99.1.2/DNA Topoisomerases, Type I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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