Document Detail


Mechanism of gene expression of Arabidopsis glutathione S-transferase, AtGST1, and AtGST11 in response to aluminum stress.
MedLine Citation:
PMID:  15047894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gene expression of two Al-induced Arabidopsis glutathione S-transferase genes, AtGST1 and AtGST11, was analyzed to investigate the mechanism underlying the response to Al stress. An approximately 1-kb DNA fragment of the 5'-upstream region of each gene was fused to a beta-glucuronidase (GUS) reporter gene (pAtGST1::GUS and pAtGST11::GUS) and introduced into Arabidopsis ecotype Landsberg erecta. The constructed transgenic lines showed a time-dependent gene expression to a different degree in the root and/or leaf by Al stress. The pAtGST1::GUS gene was induced after a short Al treatment (maximum expression after a 2-h exposure), while the pAtGST11::GUS gene was induced by a longer Al treatment (approximately 8 h for maximum expression). Since the gene expression was observed in the leaf when only the root was exposed to Al stress, a signaling system between the root and shoot was suggested in Al stress. A GUS staining experiment using an adult transgenic line carrying the pAtGST11::GUS gene supported this suggestion. Furthermore, Al treatment simultaneously with various Ca depleted conditions in root region enhanced the gene expression of the pAtGST11::GUS in the shoot region. This result suggested that the degree of Al toxicity in the root reflects the gene response of pAtGST11::GUS in the shoot via the deduced signaling system. Both transgenic lines also showed an increase of GUS activity after cold stress, heat stress, metal toxicity, and oxidative damages, suggesting a common induction mechanism in response to the tested stresses including Al stress.
Authors:
Bunichi Ezaki; Masakatsu Suzuki; Hirotoshi Motoda; Masako Kawamura; Susumu Nakashima; Hideaki Matsumoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-03-26
Journal Detail:
Title:  Plant physiology     Volume:  134     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-15     Completed Date:  2004-08-19     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1672-82     Citation Subset:  IM    
Affiliation:
Research Institute for Bioresources, Okayama University, 2-20-1, Chuou, Kurashiki, Okayama 710-0046, Japan. bezaki@rib.okayama-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / drug effects*
Aluminum / toxicity*
Arabidopsis / drug effects,  enzymology,  genetics*
Arabidopsis Proteins / genetics*,  metabolism
Base Sequence
Calcium / pharmacology
Enzyme Induction / drug effects,  genetics
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Glucuronidase / genetics,  metabolism
Glutathione Transferase / genetics*,  metabolism
Molecular Sequence Data
Plant Leaves / drug effects,  enzymology,  genetics
Plant Roots / drug effects,  enzymology,  genetics
Plant Shoots / drug effects,  enzymology,  genetics
Plants, Genetically Modified
Promoter Regions, Genetic / genetics
Sequence Homology, Nucleic Acid
Signal Transduction / drug effects,  genetics
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 7429-90-5/Aluminum; 7440-70-2/Calcium; EC 2.5.1.18/GST1 protein, Arabidopsis; EC 2.5.1.18/GST11 protein, Arabidopsis; EC 2.5.1.18/Glutathione Transferase; EC 3.2.1.31/Glucuronidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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