| Mechanism of endothelin 1 production in the cochlea of rats. | |
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MedLine Citation:
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PMID: 11174056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to elucidate the mechanism of endothelin 1 (ET1) production in the cochlea of rats. Animals were anesthetized with pentobarbital sodium intraperitoneally and sacrificed by cardiac perfusion with warm saline followed by periodate-lysine-paraformaldehyde. The temporal bones and kidneys were fixed overnight in the same fixative, decalcified with 5% EDTA and embedded in paraffin. Immunohistochemical staining was performed using the labeled streptavidin biotin method to detect the localization of big endothelin 1 (BET1), ET1 and endothelin-converting enzyme 1 (ECE1). Immunoreactivity for BET1, ET1 and ECE1 was localized to the small vessels of the bony labyrinth adjacent to the spiral ligament. Immunohistochemistry for ET1 and BET1 was also localized to the spiral ligament. These results suggest that ET1 may be produced in the small vessels of the bony labyrinth adjacent to the spiral ligament and may play an important role in the cochlear function. |
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Authors:
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K Jinnouchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: ORL; journal for oto-rhino-laryngology and its related specialties Volume: 63 ISSN: 0301-1569 ISO Abbreviation: ORL J. Otorhinolaryngol. Relat. Spec. Publication Date: 2001 Jan-Feb |
Date Detail:
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Created Date: 2001-02-22 Completed Date: 2001-07-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0334721 Medline TA: ORL J Otorhinolaryngol Relat Spec Country: Switzerland |
Other Details:
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Languages: eng Pagination: 6-11 Citation Subset: IM |
Copyright Information:
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Copyright 2001 S. Karger AG, Basel |
Affiliation:
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Department of Otolaryngology, Nippon Medical School, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid Endopeptidases / biosynthesis Cochlea / metabolism* Ear, Inner / blood supply Endothelin-1 / biosynthesis* Endothelins / biosynthesis Female Immunohistochemistry Metalloendopeptidases Protein Precursors / biosynthesis Rats Spiral Lamina / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Endothelin-1; 0/Endothelins; 0/Protein Precursors; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.71/endothelin-converting enzyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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