Document Detail

Mechanism and dynamics of breakage of fluorescent microtubules.
MedLine Citation:
PMID:  16387782     Owner:  NLM     Status:  MEDLINE    
The breakage of fluorescence-labeled microtubules under irradiation of excitation light is found in our experiments. Its mechanism is studied. The results indicate that free radicals are the main reason for the photosensitive breakage. Furthermore, the mechanical properties of the microtubules are probed with a dual-optical tweezers system. It is found that the fluorescence-labeled microtubules are much easier to extend compared with those without fluorescence. Such microtubules can be extended by 30%, and the force for breaking them up is only several piconewtons. In addition, we find that the breakup of the protofilaments is not simultaneous but step-by-step, which further confirms that the interaction between protofilaments is fairly weak.
Honglian Guo; Chunhua Xu; Chunxiang Liu; E Qu; Ming Yuan; Zhaolin Li; Bingying Cheng; Daozhong Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-30
Journal Detail:
Title:  Biophysical journal     Volume:  90     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-02     Completed Date:  2006-04-27     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2093-8     Citation Subset:  IM    
Optical Physics Laboratory, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Brain Chemistry
Dose-Response Relationship, Radiation
Elasticity / radiation effects
Micromanipulation / methods
Microscopy, Fluorescence / methods*
Microtubules / physiology*,  radiation effects,  ultrastructure*
Protein Conformation / radiation effects
Radiation Dosage
Stress, Mechanical
Tubulin / physiology*,  radiation effects,  ultrastructure*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Classical nucleation theory of virus capsids.
Next Document:  In situ background estimation in quantitative fluorescence imaging.