Document Detail


Mechanism of dilation to reactive oxygen species in human coronary arterioles.
MedLine Citation:
PMID:  14613909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We tested whether reactive oxygen species (ROS) generated from treatment with xanthine (XA) and xanthine oxidase (XO) alter vascular tone of human coronary arterioles (HCA). Fresh human coronary arterioles (HCA) from right atrial appendages were cannulated for video microscopy. ROS generated by XA (10(-4) M) + XO (10 mU/ml) dilated HCA (99 +/- 1%, 20 min after application of XA/XO). This dilation was not affected by denudation or superoxide dismutase (150 U/ml). Catalase (500 U/ml or 5,000 U/ml) attenuated the dilation early on, but a significant latent vasodilation appeared after 5 min peaking at 20 min (51 +/- 1%, 20 min after application of XA/XO + 500 U/ml catalase, P < 0.01 vs. control). KCl (40 mM) reduced the early and sustained vasodilation to XA/XO in the absence of catalase but 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 5 x 10(-5) M), diethyldithiocarbamate trihydrate (DDC, 10(-2) M), and deferoxamine (DFX, 10(-3) M) had no effect. In contrast, the catalase-resistant vasodilation was significantly attenuated by DDC, ODQ, and DFX as well as polyethylene-glycolated catalase (5,000 U/ml), but KCl had no effect. Confocal microscopy revealed that even in the presence of catalase, 2',7'-dichlorodihydrofluoresein diacetate fluorescence was observed in the vascular smooth muscle, but this was abolished by DDC. These data indicate that the exogenously generated superoxide anion (O2-*) by XA/XO is spontaneously converted to H2O2, which dilates HCA through vascular smooth muscle hyperpolarization. O2-* is also converted to H2O2 likely by superoxide dismustase within vascular cells and dilates HCA through a different pathway involving the activation of guanylate cyclase. These findings suggest that exogenously and endogenously produced H2O2 may elicit vasodilation by different mechanisms.
Authors:
Atsushi Sato; Ichiro Sakuma; David D Gutterman
Related Documents :
12003829 - Nitric oxide production is maintained in exercising swine with chronic left ventricular...
10444489 - Ischemic muscle chemoreflex response elevates blood flow in nonischemic exercising huma...
24027179 - Is there an optimal time for warfighters to supplement with protein?
9246389 - Brachial arterial blood flow during static handgrip exercise of short duration at varyi...
2390979 - Dependence on exercise intensity of changes in electrolyte secretion from the skin samp...
2163419 - Adrenaline facilitates neurogenic vasoconstriction in borderline hypertensive subjects.
17436179 - Exercise program implementation proves not feasible during acute care hospitalization.
21688869 - Carbohydrate ingestion during team games exercise: current knowledge and areas for futu...
23680679 - Effects of early exercise engagement on vascular risk in patients with transient ischem...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  285     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-17     Completed Date:  2003-12-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H2345-54     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Cardiovascular Center, and Veterans Affair Medical Center, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Arterioles / physiology
Coronary Vessels / physiology*
Humans
Hydrogen Peroxide / metabolism
Reactive Oxygen Species / metabolism*
Signal Transduction / drug effects,  physiology
Superoxide Dismutase / metabolism
Superoxides / metabolism
Vasodilation / drug effects,  physiology*
Xanthine / pharmacology
Xanthine Oxidase / pharmacology
Grant Support
ID/Acronym/Agency:
HL-65203/HL/NHLBI NIH HHS; HL-68769/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 11062-77-4/Superoxides; 69-89-6/Xanthine; 7722-84-1/Hydrogen Peroxide; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hydrogen peroxide induces endothelium-dependent and -independent coronary arteriolar dilation: role ...
Next Document:  Assessment of the ultrastructural and proliferative properties of human embryonic stem cell-derived ...