Document Detail

Mechanism for differential sensitivity of the chromosome and growth cycles of mammalian cells to the rate of protein synthesis.
MedLine Citation:
PMID:  3837839     Owner:  NLM     Status:  MEDLINE    
It has been documented widely that when the generation times of eucaryotic cells are lengthened by slowing the rate of protein synthesis, the duration of the chromosome cycle (S, G2, and M phases) remains relatively invariant. Paradoxically, when the growth of exponentially growing cultures of CHO cells is partially inhibited with inhibitors of protein synthesis, the immediate effect is a proportionate reduction in the rate of total protein, histone protein, and DNA synthesis. However, on further investigation it was found that over the next 2 h the rates of histone protein and DNA synthesis recover, in some cases completely to the uninhibited rate, while the synthesis rates of other proteins do not recover. We called this process chromosome cycle compensation. The amount of compensation seen in CHO cell cultures can account quantitatively for the relative invariance in the length of the chromosome cycle (S, G2, and M phases) reported for these cells. The mechanism for this compensation involves a specific increase in the levels of histone mRNAs. An invariant chromosome cycle coupled with a lengthening growth cycle must result in a disproportionate lengthening of the G1 phase. Thus, these results suggest that chromosome cycle invariance may be due more to specific cellular compensation mechanisms rather than to the more usual interpretation involving a rate-limiting step for cell cycle progression in the G1 phase.
R S Wu; W M Bonner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  5     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-09-29     Completed Date:  1986-09-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2959-66     Citation Subset:  IM    
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MeSH Terms
Cell Cycle* / drug effects
Cell Line
Chromosomes / physiology*
Cycloheximide / pharmacology
DNA Replication / drug effects
Histones / biosynthesis,  genetics
Hydroxyurea / pharmacology
Protein Biosynthesis* / drug effects
Reg. No./Substance:
0/Histones; 127-07-1/Hydroxyurea; 66-81-9/Cycloheximide

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