Document Detail

Mechanism of complement-induced cell lysis. Demonstration of a three-step mechanism of EAC1-8 cell lysis by C9 and of a non-osmotic swelling of erythrocytes.
MedLine Citation:
PMID:  809505     Owner:  NLM     Status:  MEDLINE    
Sheep erythrocytes were sized by an electrical method during lysis by antibody and complement. No volume changes were observed upon to the EAC1-8 cell intermediate. Three steps could be distinguished in the reaction of C9 with EAC1-8 cells: fixation of C9 to EAC1-8 cells, a process which proceeds also at 0 degrees C; temperature-dependent swelling of the EAC1-9 cells; and lysis of the cells. We could show that swelling of the erythrocytes induced by the complement reaction is independent of protein osmotic forces and is caused by an alteration of the erythrocyte membrane.
G Valet; W Opferkuch
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  115     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1975-12-20     Completed Date:  1975-12-20     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1028-33     Citation Subset:  AIM; IM    
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MeSH Terms
Antigen-Antibody Reactions
Cell Membrane / immunology
Complement System Proteins / metabolism*
Edetic Acid / metabolism
Erythrocyte Count
Erythrocytes / immunology*
Guinea Pigs
Hemoglobins / analysis
Phenanthrolines / metabolism
Serum Albumin, Bovine / metabolism
Reg. No./Substance:
0/Buffers; 0/Hemoglobins; 0/Phenanthrolines; 0/Serum Albumin, Bovine; 60-00-4/Edetic Acid; 9007-36-7/Complement System Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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