Document Detail


Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces.
MedLine Citation:
PMID:  7772669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poly(N-isopropylacrylamide) (PIPAAm), exhibiting a lower critical solution temperature (LCST) at 25 degrees C in physiological phosphate buffered saline solution (pH 7.4) and at 32 degrees C in pure water, was grafted onto the surfaces of commercial polystyrene cell culture dishes. This PIPAAm-grafted surface exhibited hydrophobic surface properties at temperatures over the LCST and hydrophilic surface properties below the LCST. Endothelial cells and hepatocytes attached and proliferated on PIPAAm-grafted surfaces at 37 degrees C, above the LCST. The cultured cells were readily detached from these surfaces by lowering the incubation temperature without the usual damage associated with trypsinization. In this case, the optimum temperature for cell detachment was 10 degrees C for hepatocytes and 20 degrees C for endothelial cells. Cell detachment was partially inhibited by sodium azide treatment, suggesting that cell metabolism directly affects cell detachment. Morphological changes of the adherent cells during cell detachment experiments indicated further involvement of active cellular metabolic processes. Cells detached from hydrophobic-hydrophilic PIPAAm surfaces not only via reduced cell-surface interactions caused by the spontaneous hydration of grafted PIPAAm chains, but also by active cell morphological changes which were a function of cell metabolism.
Authors:
T Okano; N Yamada; M Okuhara; H Sakai; Y Sakurai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  16     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-07-13     Completed Date:  1995-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  297-303     Citation Subset:  IM    
Affiliation:
Institute of Biomedical Engineering, Tokyo Women's Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / metabolism*
Animals
Aorta, Thoracic / cytology,  drug effects,  metabolism
Azides / toxicity
Cattle
Cell Adhesion / drug effects,  physiology
Cell Division / drug effects,  physiology
Cells, Cultured
DNA / biosynthesis
Endothelium, Vascular / cytology*,  metabolism
Hydrogen-Ion Concentration
Liver / cytology*,  metabolism
Male
Microscopy, Electron, Scanning
Microscopy, Phase-Contrast
Mutagens / toxicity
Polystyrenes / chemistry
Rats
Rats, Wistar
Sodium Azide
Temperature
Trypsin / chemistry
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Azides; 0/Mutagens; 0/Polystyrenes; 25189-55-3/poly-N-isopropylacrylamide; 26628-22-8/Sodium Azide; 9007-49-2/DNA; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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