| Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces. | |
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MedLine Citation:
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PMID: 7772669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly(N-isopropylacrylamide) (PIPAAm), exhibiting a lower critical solution temperature (LCST) at 25 degrees C in physiological phosphate buffered saline solution (pH 7.4) and at 32 degrees C in pure water, was grafted onto the surfaces of commercial polystyrene cell culture dishes. This PIPAAm-grafted surface exhibited hydrophobic surface properties at temperatures over the LCST and hydrophilic surface properties below the LCST. Endothelial cells and hepatocytes attached and proliferated on PIPAAm-grafted surfaces at 37 degrees C, above the LCST. The cultured cells were readily detached from these surfaces by lowering the incubation temperature without the usual damage associated with trypsinization. In this case, the optimum temperature for cell detachment was 10 degrees C for hepatocytes and 20 degrees C for endothelial cells. Cell detachment was partially inhibited by sodium azide treatment, suggesting that cell metabolism directly affects cell detachment. Morphological changes of the adherent cells during cell detachment experiments indicated further involvement of active cellular metabolic processes. Cells detached from hydrophobic-hydrophilic PIPAAm surfaces not only via reduced cell-surface interactions caused by the spontaneous hydration of grafted PIPAAm chains, but also by active cell morphological changes which were a function of cell metabolism. |
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Authors:
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T Okano; N Yamada; M Okuhara; H Sakai; Y Sakurai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomaterials Volume: 16 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-07-13 Completed Date: 1995-07-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 297-303 Citation Subset: IM |
Affiliation:
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Institute of Biomedical Engineering, Tokyo Women's Medical College, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins
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metabolism* Animals Aorta, Thoracic / cytology, drug effects, metabolism Azides / toxicity Cattle Cell Adhesion / drug effects, physiology Cell Division / drug effects, physiology Cells, Cultured DNA / biosynthesis Endothelium, Vascular / cytology*, metabolism Hydrogen-Ion Concentration Liver / cytology*, metabolism Male Microscopy, Electron, Scanning Microscopy, Phase-Contrast Mutagens / toxicity Polystyrenes / chemistry Rats Rats, Wistar Sodium Azide Temperature Trypsin / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Azides; 0/Mutagens; 0/Polystyrenes; 25189-55-3/poly-N-isopropylacrylamide; 26628-22-8/Sodium Azide; 9007-49-2/DNA; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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