Document Detail


Mechanism of cell damage by ultrasound in combination with hematoporphyrin.
MedLine Citation:
PMID:  2172198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism of cell damage by ultrasound in combination with hematoporphyrin was studied. Mouse sarcoma 180 cell suspensions were exposed to ultrasound for up to 60 s in the presence and absence of hematoporphyrin, with and without active oxygen scavengers. The cell damage enhancement by hematoporphyrin was suppressed by adding histidine but not by mannitol. The enhancement was doubled in rate by substitution of deuterium oxide medium for normal water. Sonoluminescence was produced in a saline solution under similar acoustic conditions and observed to have spectral components that can excite hematoporphyrin molecules. These results suggest that cell damage enhancement is probably mediated via singlet oxygen generated by ultrasonically activated hematoporphyrin.
Authors:
S Umemura; N Yumita; R Nishigaki; K Umemura
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  81     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-12-13     Completed Date:  1990-12-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  962-6     Citation Subset:  IM    
Affiliation:
Central Research Laboratory, Hitachi, Ltd., Tokyo.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects*
Deuterium
Deuterium Oxide
Free Radicals
Hematoporphyrins / toxicity*
Histidine / pharmacology
Luminescent Measurements
Mannitol / pharmacology
Sarcoma, Experimental / drug therapy
Superoxide Dismutase / metabolism
Temperature
Tumor Cells, Cultured
Ultrasonics*
Water
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Hematoporphyrins; 69-65-8/Mannitol; 71-00-1/Histidine; 7732-18-5/Water; 7782-39-0/Deuterium; 7789-20-0/Deuterium Oxide; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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