| Mechanism of arachidonic acid release in human polymorphonuclear leukocytes. | |
| | |
MedLine Citation:
|
PMID: 6402027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previous studies have demonstrated that [3H]arachidonic acid is released from prelabeled human neutrophil phospholipids when the cells are stimulated by calcium ionophore A23187 or by opsonized zymosan. Neither lysophospholipid generated by phospholipase A2 activity, diacylglycerol nor monoacylglycerol produced via phospholipase C/diacylglycerol lipase action have been identified following neutrophil challenge. The inability to detect any intermediates during the release of arachidonate is due to either rapid reacylation of lysophospholipid or conversion of diacylglycerol (monoacylglycerol) to cellular acylglycerols. The addition of exogenous [14C]fatty acid at the time of challenge was employed to determine the involvement of either phospholipase A2 or phospholipase C activities. Neutrophil stimulation with calcium ionophore A23187 resulted in an incorporation of exogenous [14C]arachidonate into phosphatidylinositol and phosphatidylcholine, those phospholipids which specifically release arachidonate. When the saturated fatty acid, [14C]stearate, replaced [14C]arachidonate, very little [14C]fatty acid was incorporated into any of the phospholipid species. Lipid phosphorus measurements revealed no significant mass change in any phospholipid class following ionophore challenge. Production of [14C]phosphatidic acid was not detected, as would be expected if diacylglycerol kinase and de novo phospholipid metabolism were significantly involved. |
| | |
Authors:
|
C E Walsh; L R Dechatelet; F H Chilton; R L Wykle; M Waite |
Related Documents
:
|
12179967 - Phospholipid signalling in plant defence. 1095777 - Phospholipase activity in bacteriophage-infected escherichia. ii. activation of phospho... 6894247 - Influence of dicarboxylic phosphatidylcholines on the stability and phase transition of... 7794957 - Interaction of free fatty acids with phospholipid bilayers. 2782107 - Free fatty acid determination by peroxidase catalysed luminol chemiluminescence. 7683207 - Sulfonic acid polymers are potent inhibitors of hiv-1 induced cytopathogenicity and the... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 750 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1983 Jan |
Date Detail:
|
Created Date: 1983-04-15 Completed Date: 1983-04-15 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 32-40 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Arachidonic Acid Arachidonic Acids / blood* Calcimycin / pharmacology Carbon Radioisotopes / diagnostic use Humans Kinetics Neutrophils / drug effects, metabolism* Phospholipids / blood* Stearic Acids / blood Tritium / diagnostic use Zymosan / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
AI-10732/AI/NIAID NIH HHS; AI-17287/AI/NIAID NIH HHS; AM-11799/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Arachidonic Acids; 0/Carbon Radioisotopes; 0/Phospholipids; 0/Stearic Acids; 10028-17-8/Tritium; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 57-11-4/stearic acid; 9010-72-4/Zymosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phosphatidylinositol and phosphatidic acid metabolism in rat pancreatic islets in response to neurot...
Next Document: Multienzyme complexes of fatty acid oxidation from Escherichia coli K12 and from a mutant with a def...