Document Detail

Mechanism of antiulcer effect of Neem (Azadirachta indica) leaf extract: effect on H+-K+-ATPase, oxidative damage and apoptosis.
MedLine Citation:
PMID:  15265317     Owner:  NLM     Status:  MEDLINE    
The mechanism of the antiulcer effect of Neem leaf aqueous extract to block gastric lesions in rat has been studied with emphasis on acid secretion, oxidative damage and apoptosis. The extract dose-dependently inhibits gastric lesions induced by restraint-cold stress, indomethacin and ethanol. In stress ulcer model, it is more effective than ranitidine but less effective than omeprazole. It also dose-dependently blocks pylorus ligation and mercaptomethylimidazole-induced acid secretion. In the pylorus-ligation model, it is less effective than omeprazole but as effective as ranitidine. It inhibits H+-K+-ATPase activity in vitro in concentration-dependent manner to inhibit acid secretion. Oxidative membrane damage by hydroxyl radical (*OH) as measured by lipid peroxidation in stress ulcer is significantly blocked by leaf extract. Stress-induced apoptotic DNA fragmentation is also protected. The extract also prevents *OH-mediated mucosal DNA damage in vitro by scavenging the *OH. Neem leaf extract, thus, offers antiulcer activity by blocking acid secretion through inhibition of H+-K+-ATPase and by preventing oxidative damage and apoptosis.
Ishita Chattopadhyay; Bithi Nandi; Ratna Chatterjee; Kaushik Biswas; Uday Bandyopadhyay; Ranajit K Banerjee
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammopharmacology     Volume:  12     ISSN:  0925-4692     ISO Abbreviation:  Inflammopharmacology     Publication Date:  2004  
Date Detail:
Created Date:  2004-07-21     Completed Date:  2005-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9112626     Medline TA:  Inflammopharmacology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-76     Citation Subset:  IM    
Department of Physiology, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata-700 032, India.
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MeSH Terms
Anti-Ulcer Agents / isolation & purification,  pharmacology*,  therapeutic use
Apoptosis / drug effects*,  physiology
Dose-Response Relationship, Drug
H(+)-K(+)-Exchanging ATPase / antagonists & inhibitors*,  metabolism
Oxidative Stress / drug effects*,  physiology
Plant Extracts / isolation & purification,  pharmacology,  therapeutic use
Plant Leaves
Rats, Sprague-Dawley
Stomach Ulcer / drug therapy,  enzymology
Reg. No./Substance:
0/Anti-Ulcer Agents; 0/Plant Extracts; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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