Document Detail


Mechanism of antiulcer effect of Neem (Azadirachta indica) leaf extract: effect on H+-K+-ATPase, oxidative damage and apoptosis.
MedLine Citation:
PMID:  15265317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism of the antiulcer effect of Neem leaf aqueous extract to block gastric lesions in rat has been studied with emphasis on acid secretion, oxidative damage and apoptosis. The extract dose-dependently inhibits gastric lesions induced by restraint-cold stress, indomethacin and ethanol. In stress ulcer model, it is more effective than ranitidine but less effective than omeprazole. It also dose-dependently blocks pylorus ligation and mercaptomethylimidazole-induced acid secretion. In the pylorus-ligation model, it is less effective than omeprazole but as effective as ranitidine. It inhibits H+-K+-ATPase activity in vitro in concentration-dependent manner to inhibit acid secretion. Oxidative membrane damage by hydroxyl radical (*OH) as measured by lipid peroxidation in stress ulcer is significantly blocked by leaf extract. Stress-induced apoptotic DNA fragmentation is also protected. The extract also prevents *OH-mediated mucosal DNA damage in vitro by scavenging the *OH. Neem leaf extract, thus, offers antiulcer activity by blocking acid secretion through inhibition of H+-K+-ATPase and by preventing oxidative damage and apoptosis.
Authors:
Ishita Chattopadhyay; Bithi Nandi; Ratna Chatterjee; Kaushik Biswas; Uday Bandyopadhyay; Ranajit K Banerjee
Related Documents :
2564767 - Inhibition of the stimulated canine exocrine pancreas by amino acids and fat.
9100287 - Characterization of somatostatin receptor subtypes mediating inhibition of nutrient-sti...
6259537 - Substituted benzimidazoles inhibit gastric acid secretion by blocking (h+ + k+)atpase.
16651247 - Methyl jasmonate treatment of mature norway spruce (picea abies) trees increases the ac...
7202647 - Action of gaba and glycine on the membrane potential of culture astrocytes and the extr...
18964947 - Direct spectrophotometric determination of nd and er in mixed rare earths with 8-hydrox...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammopharmacology     Volume:  12     ISSN:  0925-4692     ISO Abbreviation:  Inflammopharmacology     Publication Date:  2004  
Date Detail:
Created Date:  2004-07-21     Completed Date:  2005-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9112626     Medline TA:  Inflammopharmacology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-76     Citation Subset:  IM    
Affiliation:
Department of Physiology, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata-700 032, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Ulcer Agents / isolation & purification,  pharmacology*,  therapeutic use
Apoptosis / drug effects*,  physiology
Azadirachta*
Dose-Response Relationship, Drug
H(+)-K(+)-Exchanging ATPase / antagonists & inhibitors*,  metabolism
Male
Oxidative Stress / drug effects*,  physiology
Plant Extracts / isolation & purification,  pharmacology,  therapeutic use
Plant Leaves
Rats
Rats, Sprague-Dawley
Stomach Ulcer / drug therapy,  enzymology
Chemical
Reg. No./Substance:
0/Anti-Ulcer Agents; 0/Plant Extracts; EC 3.6.1.10/H(+)-K(+)-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Anti-inflammatory properties of BHUx, a polyherbal formulation to prevent atherosclerosis.
Next Document:  Role of epidermal growth factor receptor transactivation in PPAR gamma-dependent suppression of Heli...