Document Detail

Mechanism of the antihypertensive effect of stevioside in anesthetized dogs.
MedLine Citation:
PMID:  12444299     Owner:  NLM     Status:  MEDLINE    
Stevioside is a sweet-tasting glycoside isolated from the leaves of Stevia rebaudiana. It has been used as a noncaloric sugar substitute in Japan and Brazil for decades. Previous studies have shown that it lowered blood pressure in spontaneously hypertensive rats by intravenous injection. This study was designed to evaluate the hypotensive effect of stevioside in dogs and to define the underlying mechanism. After nasogastric administration of stevioside powder (200 mg/kg), the blood pressure of healthy mongrel dogs began to significantly decrease at 60 min and returned to baseline level at 180 min. The reduction of blood pressure was more rapid (at 5-10 min) and effective after intravenous injection. However, no significant change of blood pressure was noted after injection through left vertebral artery, implicating that the hypotensive effect is not related to the central nervous system. Stevioside also showed significant hypotensive effects in renal hypertensive dogs, in a dose-dependent manner. In cultured rat aortic smooth muscle cells (A7r5 cell line), stevioside can dose-dependently inhibit the stimulatory effects of vasopressin and phenylephrine on intracellular Ca(2+) in a calcium-containing medium. However, no intracellular Ca(2+) inhibitory effect was observed in calcium-free medium, implicating that stevioside may inhibit the Ca(2+) influx from extracellular fluid. Our present data show that stevioside did not influence the calcium ionophore (A23187) induced Ca(2+) influx, indicating that the antagonistic effect was through Ca(2+) channels. This study confirmed that stevioside is an effective antihypertensive natural product, and its hypotensive mechanism may be probably due to inhibition of the Ca(2+) influx.
Ju-Chi Liu; Pai-Keng Kao; Paul Chan; Yung-Ho Hsu; Chun-Cheng Hou; Gi-Shih Lien; Min-Hsiung Hsieh; Yi-Jen Chen; Juei-Tang Cheng
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Pharmacology     Volume:  67     ISSN:  0031-7012     ISO Abbreviation:  Pharmacology     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-11-21     Completed Date:  2003-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0152016     Medline TA:  Pharmacology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  14-20     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Department of Medicine, Taipei Medical University--Wan Fang Hospital, Taipei, Taiwan.
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MeSH Terms
Administration, Intranasal
Antihypertensive Agents / administration & dosage,  pharmacology*
Aorta / metabolism
Blood Pressure / drug effects*,  physiology
Calcium / metabolism*
Cell Line
Cytosol / metabolism
Diterpenes / administration & dosage,  pharmacology*
Diterpenes, Kaurane*
Dose-Response Relationship, Drug
Glucosides / administration & dosage,  pharmacology*
Hypertension, Renal / drug therapy,  metabolism,  physiopathology*
Injections, Intra-Arterial
Injections, Intravenous
Muscle, Smooth, Vascular / metabolism*
Renal Artery / physiology
Time Factors
Vertebral Artery / physiology
Reg. No./Substance:
0/Antihypertensive Agents; 0/Diterpenes; 0/Diterpenes, Kaurane; 0/Glucosides; 57817-89-7/stevioside; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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