| Mechanism of adenine toxicity in Escherichia coli. | |
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MedLine Citation:
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PMID: 6801015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism of adenine toxicity in an hpt gpt strain of Escherichia coli that is extremely sensitive to adenine inhibition was investigated. Adenine-resistant derivatives had secondary mutations in adeninephosphoribosyltransferase or the purR repressor. Growth studies with various purine salvage pathway mutants and the ability of guanosine to prevent adenine toxicity indicated that adenine exerts its toxic effects by depleting guanine nucleotide pools. In the presence of adenine, ATP pools increased twofold in wild-type cells and stabilized after 5 min. In contrast, ATP pools continued to rise in hpt gpt cells up to 25 min and increased sevenfold after adenine addition. hpt gpt cells were shown to have higher levels of adeninephosphoribosyltransferase than did the wild-type cells. In response to adenine addition, GTP pools dropped three- to fourfold in all strains tested. Although GTP levels returned to near normal values in wild-type cells after 35 min, no restoration of GTP pools was observed in the hpt gpt strain during this period. Measurements of guanine pools before and after the addition of adenine indicated that guaninephosphoribosyltransferase plays an important role in maintaining GTP pools by converting the free guanine to GMP during guanine nucleotide depletion. |
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Authors:
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R A Levine; M W Taylor |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 149 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1982 Mar |
Date Detail:
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Created Date: 1982-05-21 Completed Date: 1982-05-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 923-30 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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pharmacology* Adenine Phosphoribosyltransferase / metabolism Adenosine Triphosphate / metabolism Escherichia coli / drug effects*, metabolism Guanosine / pharmacology Guanosine Triphosphate / metabolism* Hypoxanthine Phosphoribosyltransferase / metabolism Mutation Pentosyltransferases / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AM 25498/AM/NIADDK NIH HHS; GM 18924/GM/NIGMS NIH HHS; S07 RR 7031/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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118-00-3/Guanosine; 56-65-5/Adenosine Triphosphate; 73-24-5/Adenine; 86-01-1/Guanosine Triphosphate; EC 2.4.2.-/Pentosyltransferases; EC 2.4.2.7/Adenine Phosphoribosyltransferase; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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