Document Detail

Mechanism of TNF-α-induced migration and hepatocyte growth factor production in human mesenchymal stem cells.
MedLine Citation:
PMID:  20533298     Owner:  NLM     Status:  MEDLINE    
Accumulating evidence suggests that mesenchymal stem cells (MSCs) may decrease destructive inflammation and reduce tissue loss. Tumor necrosis factor-α (TNF-α) plays a central role in induction of proinflammatory signaling and paradoxically activates intracellular anti-inflammatory survival pathways. In this study, we investigated whether TNF-α could induce a chemotactic effect on human MSCs and stimulate their production of anti-inflammatory factors in vitro, as well as determined mechanisms that mediated this effect. Migration assays demonstrated that TNF-α had a chemotactic effect on MSCs. TNF-α increased both hepatocyte growth factor (HGF) mRNA expression in MSCs and HGF secretion in conditioned medium. These effects were dependent on the p38 MAPK and PI3K/Akt, but not JNK and ERK signaling pathways. Furthermore, these effects were inhibited by a specific neutralizing antibody to TNF receptor II, but not TNF receptor I. We conclude that TNF-α can enhance human MSCs migration and stimulate their production of HGF. These effects are mediated via a specific TNF receptor and signaling pathways.
Aibin Zhang; Yan Wang; Zhou Ye; Haiyang Xie; Lin Zhou; Shusen Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  111     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  469-75     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
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MeSH Terms
Cell Movement / drug effects
Cells, Cultured
Culture Media, Conditioned
Hepatocyte Growth Factor / biosynthesis*
Mesenchymal Stem Cells
RNA, Messenger / analysis
Receptors, Tumor Necrosis Factor
Signal Transduction
Tumor Necrosis Factor-alpha / pharmacology*
Reg. No./Substance:
0/Culture Media, Conditioned; 0/RNA, Messenger; 0/Receptors, Tumor Necrosis Factor; 0/Tumor Necrosis Factor-alpha; 67256-21-7/Hepatocyte Growth Factor

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