| Mechanism of TNF-α-induced migration and hepatocyte growth factor production in human mesenchymal stem cells. | |
| | |
MedLine Citation:
|
PMID: 20533298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Accumulating evidence suggests that mesenchymal stem cells (MSCs) may decrease destructive inflammation and reduce tissue loss. Tumor necrosis factor-α (TNF-α) plays a central role in induction of proinflammatory signaling and paradoxically activates intracellular anti-inflammatory survival pathways. In this study, we investigated whether TNF-α could induce a chemotactic effect on human MSCs and stimulate their production of anti-inflammatory factors in vitro, as well as determined mechanisms that mediated this effect. Migration assays demonstrated that TNF-α had a chemotactic effect on MSCs. TNF-α increased both hepatocyte growth factor (HGF) mRNA expression in MSCs and HGF secretion in conditioned medium. These effects were dependent on the p38 MAPK and PI3K/Akt, but not JNK and ERK signaling pathways. Furthermore, these effects were inhibited by a specific neutralizing antibody to TNF receptor II, but not TNF receptor I. We conclude that TNF-α can enhance human MSCs migration and stimulate their production of HGF. These effects are mediated via a specific TNF receptor and signaling pathways. |
| | |
Authors:
|
Aibin Zhang; Yan Wang; Zhou Ye; Haiyang Xie; Lin Zhou; Shusen Zheng |
Related Documents
:
|
22386198 - Peripheral innate immune challenge exaggerated microglia activation, increased the numb... 22331068 - Neonatal rhinovirus infection induces mucous metaplasia and airways hyperresponsiveness. 10919278 - Dexamethasone suppresses tumor necrosis factor-alpha-induced apoptosis in osteoblasts: ... 22314318 - Effect of medroxyprogesterone on development of pentylenetetrazole-induced kindling in ... 8327658 - Thrombopoietin from human embryonic kidney cells stimulates an increase in megakaryocyt... 16420598 - Prophylactic use of liposomized tuftsin enhances the susceptibility of candida albicans... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 111 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-27 Completed Date: 2011-01-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 469-75 Citation Subset: IM |
Copyright Information:
|
© 2010 Wiley-Liss, Inc. |
Affiliation:
|
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Movement
/
drug effects Cells, Cultured Culture Media, Conditioned Hepatocyte Growth Factor / biosynthesis* Humans Mesenchymal Stem Cells RNA, Messenger / analysis Receptors, Tumor Necrosis Factor Signal Transduction Tumor Necrosis Factor-alpha / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media, Conditioned; 0/RNA, Messenger; 0/Receptors, Tumor Necrosis Factor; 0/Tumor Necrosis Factor-alpha; 67256-21-7/Hepatocyte Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Activin A and bone metastasis.
Next Document: LPA rescues ER stress-associated apoptosis in hypoxia and serum deprivation-stimulated mesenchymal s...