| Mechanism of free Zn(2+) enhancing inhibitory effects of EGCG on the growth of PC-3 cells: interactions with mitochondria. | |
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MedLine Citation:
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PMID: 19326061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Green tea and its major constituent epigallocatechin gallate (EGCG) are known for their chemopreventive effects including those against prostate cancer, which could be mediated by metal ions. Zn(2+) is an essential trace element that is required for human health and plays an important role in the normal function of the prostate gland. In the present study, the effect of EGCG on cell membrane and mitochondria of PC-3 (prostate carcinoma) cells in the presence and absence of Zn(2+) was studied. These studies revealed that EGCG, Zn(2+), or EGCG + Zn(2+) affected the morphology of PC-3 cells and induced apoptosis in PC-3 cells. It was observed that effects of treatment with EGCG, Zn(2+), or EGCG + Zn(2+)on mitochondria showed EGCG + Zn(2+) > Zn(2+) > EGCG, including cytochrome C release from the intermembrane space into the cytosol, inhibited the synthesis of ATP, loss of mitochondrial membrane potential, and activation of caspase-9. However, the order of effect on depressing membrane fluidity of PC-3 cells was EGCG > EGCG + Zn(2+) > Zn(2+). In summary, these findings suggest that EGCG, Zn(2+), and EGCG + Zn(2+) induce necrosis or apoptosis of PC-3 cells through mitochondria-mediated apoptotic pathway and free Zn(2+)-enhanced effects of EGCG on PC-3 cells due to its interactions with mitochondria. |
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Authors:
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Junguo Yang; Haining Yu; Shili Sun; Lancui Zhang; Undurti N Das; Hui Ruan; Guoqing He; Shengrong Shen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-27 |
Journal Detail:
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Title: Biological trace element research Volume: 131 ISSN: 1559-0720 ISO Abbreviation: Biol Trace Elem Res Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-02 Completed Date: 2010-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7911509 Medline TA: Biol Trace Elem Res Country: United States |
Other Details:
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Languages: eng Pagination: 298-310 Citation Subset: IM |
Affiliation:
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Hua Jia Chi Campus, Zhejiang University, Hangzhou, 310029, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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biosynthesis Apoptosis / drug effects Caspase 9 / metabolism Catechin / administration & dosage, analogs & derivatives* Cell Division / drug effects Cell Line, Tumor Cytochromes c / metabolism Drug Synergism Humans Male Membrane Fluidity / drug effects Membrane Potential, Mitochondrial / drug effects Mitochondria / drug effects, metabolism Prostatic Neoplasms / drug therapy*, metabolism, pathology Zinc / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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154-23-4/Catechin; 56-65-5/Adenosine Triphosphate; 7440-66-6/Zinc; 9007-43-6/Cytochromes c; 989-51-5/epigallocatechin gallate; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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