Document Detail

Mechanism of free Zn(2+) enhancing inhibitory effects of EGCG on the growth of PC-3 cells: interactions with mitochondria.
MedLine Citation:
PMID:  19326061     Owner:  NLM     Status:  MEDLINE    
Green tea and its major constituent epigallocatechin gallate (EGCG) are known for their chemopreventive effects including those against prostate cancer, which could be mediated by metal ions. Zn(2+) is an essential trace element that is required for human health and plays an important role in the normal function of the prostate gland. In the present study, the effect of EGCG on cell membrane and mitochondria of PC-3 (prostate carcinoma) cells in the presence and absence of Zn(2+) was studied. These studies revealed that EGCG, Zn(2+), or EGCG + Zn(2+) affected the morphology of PC-3 cells and induced apoptosis in PC-3 cells. It was observed that effects of treatment with EGCG, Zn(2+), or EGCG + Zn(2+)on mitochondria showed EGCG + Zn(2+) > Zn(2+) > EGCG, including cytochrome C release from the intermembrane space into the cytosol, inhibited the synthesis of ATP, loss of mitochondrial membrane potential, and activation of caspase-9. However, the order of effect on depressing membrane fluidity of PC-3 cells was EGCG > EGCG + Zn(2+) > Zn(2+). In summary, these findings suggest that EGCG, Zn(2+), and EGCG + Zn(2+) induce necrosis or apoptosis of PC-3 cells through mitochondria-mediated apoptotic pathway and free Zn(2+)-enhanced effects of EGCG on PC-3 cells due to its interactions with mitochondria.
Junguo Yang; Haining Yu; Shili Sun; Lancui Zhang; Undurti N Das; Hui Ruan; Guoqing He; Shengrong Shen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-27
Journal Detail:
Title:  Biological trace element research     Volume:  131     ISSN:  1559-0720     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  298-310     Citation Subset:  IM    
Hua Jia Chi Campus, Zhejiang University, Hangzhou, 310029, People's Republic of China.
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MeSH Terms
Adenosine Triphosphate / biosynthesis
Apoptosis / drug effects
Caspase 9 / metabolism
Catechin / administration & dosage,  analogs & derivatives*
Cell Division / drug effects
Cell Line, Tumor
Cytochromes c / metabolism
Drug Synergism
Membrane Fluidity / drug effects
Membrane Potential, Mitochondrial / drug effects
Mitochondria / drug effects,  metabolism
Prostatic Neoplasms / drug therapy*,  metabolism,  pathology
Zinc / administration & dosage*
Reg. No./Substance:
154-23-4/Catechin; 56-65-5/Adenosine Triphosphate; 7440-66-6/Zinc; 9007-43-6/Cytochromes c; 989-51-5/epigallocatechin gallate; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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