Document Detail


Mechanism and Effects of Glucose Absorption during an Oral Glucose Tolerance Test Among Females and Males.
MedLine Citation:
PMID:  21147888     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: Several epidemiological studies revealed sex-specific differences during oral glucose tolerance tests (OGTTs), such as higher prevalence of glucose intolerance (i.e. increased glucose at the end of the OGTT) in females, which was not yet explained. Thus, we aimed to analyze sex-related distinctions on OGTT glucose metabolism, including gut absorption, in healthy humans. Methods: Females (n = 48) and males (n = 26) with comparable age (females, 45 ± 1 yr; males, 44 ± 2 yr) and body mass index (both, 25 ± 1 kg/m(2)) but different height (females, 166 ± 1 cm; males, 180 ± 2 cm; P < 0.000001), all normally glucose tolerant, as tested by frequently sampled, 3-h (75-g) OGTTs, underwent hyperinsulinemic [40 mU/(min · m(2))] isoglycemic clamp tests with simultaneous measurement of endogenous glucose (D-[6,6-(2)H2]glucose) production (EGP). EGP and glucose disappearance during OGTT were calculated from logarithmic relationships with clamp test insulin concentrations. After reliable model validation by double-tracer technique (r = 0.732; P < 0.007), we calculated and modeled gut glucose absorption (ABS). Results: Females showed lower (P < 0.05) fasting EGP [1.4 ± 0.1 mg/(kg · min)] than males [1.7 ± 0.1 mg/(kg · min)] but comparable whole-body insulin sensitivity in clamp tests [females, 8.1 ± 0.4 mg/(kg · min); males, 8.3 ± 0.6 mg/(kg · min)]. Plasma glucose OGTT concentrations were higher (P < 0.04) from 30-40 min in males but from 120-180 min in females. Glucose absorption rates were 21-46% increased in the initial 40 min in males but in females by 27-40% in the third hour (P < 0.05). Gut glucose half-life was markedly higher in females (79 ± 2 min) than in males (65 ± 3 min, P < 0.0001) and negatively related to body height (r = -0.481; P < 0.0001). Conclusions: This study in healthy, glucose-tolerant humans shows for the first time different ABS rates during OGTT in women and men and a negative relationship between body height and gut glucose half-life. Prolonged ABS in females might therefore contribute to higher plasma glucose concentrations at the end of OGTT.
Authors:
Christian Anderwald; Amalia Gastaldelli; Andrea Tura; Michael Krebs; Miriam Promintzer-Schifferl; Alexandra Kautzky-Willer; Marietta Stadler; Ralph A Defronzo; Giovanni Pacini; Martin G Bischof
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-8
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  -     ISSN:  1945-7197     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Division of Endocrinology and Metabolism (C.A., M.K., M.P.-S., A.K.-W., M.G.B.), Department of Internal Medicine III, Medical University of Vienna, A-1090 Vienna, Austria; Metabolic Unit (C.A., A.T., G.P.), Institute of Biomedical Engineering, National Research Council, I-35127 Padova, Italy; National Research Council Institute of Clinical Physiology (A.G.), I-56124 Pisa, Italy; Third Medical Department of Metabolic Diseases and Nephrology (M.S.) and Karl Landsteiner Institute of Metabolic Diseases and Nephrology (M.S.), Hietzing Hospital, A-1130 Vienna, Austria; Department of Medicine/Diabetes (R.A.D.), University of Texas Health Science Center, San Antonio, Texas 78207; and Medical Direction (M.G.B.), St. Elisabeth Hospital, A-1030 Vienna, Austria.
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