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Mechanism-Based Small Molecule Cross-Linkers of HECT E3 Ubiquitin Ligase - Substrate Pairs.
MedLine Citation:
PMID:  23043241     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Here we report the discovery that bifunctional thiol- and amine-reactive electrophiles serve as mechanism-based covalent cross-linkers for HECT E3 ubiquitin ligase/substrate pairs. We demonstrate that these chemical cross-linkers covalently cross-link the catalytic Cys residue of the yeast HECT E3 ubiquitin ligase Rsp5 with the Lys of the ubiquitination site in the model substrate Sic60-GFP. This work represents the first example of mechanism-based covalent cross-link of HECT E3/substrate pairs that converts transiently interacting HECT E3/substrate pairs into stable, covalently cross-linked protein complexes, thereby facilitating their subsequent isolation, identification, and study.
Authors:
Sungjin Park; Ioanna Ntai; Paul Martin Thomas; Evgeniia V Konishcheva; Neil L Kelleher; Alexander V Statsuk
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-8
Journal Detail:
Title:  Biochemistry     Volume:  -     ISSN:  1520-4995     ISO Abbreviation:  Biochemistry     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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