| Mechanical ventilation-induced oxidative stress in the diaphragm: role of heme oxygenase-1. | |
| | |
MedLine Citation:
|
PMID: 21106654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Prolonged mechanical ventilation (MV) results in a rapid onset of diaphragmatic atrophy that is primarily due to increased proteolysis. Although MV-induced protease activation can involve several factors, it is clear that oxidative stress is a required signal for protease activation in the diaphragm during prolonged MV. However, the oxidant-producing pathways in the diaphragm that contribute to MV-induced oxidative stress remain unknown. We have demonstrated that prolonged MV results in increased diaphragmatic expression of a key stress-sensitive enzyme, heme oxygenase (HO)-1. Paradoxically, HO-1 can function as either a pro-oxidant or an antioxidant, and the role that HO-1 plays in MV-induced diaphragmatic oxidative stress is unknown. We tested the hypothesis that HO-1 acts as a pro-oxidant in the diaphragm during prolonged MV. METHODS: To determine whether HO-1 functions as a pro-oxidant or an antioxidant in the diaphragm during MV, we assigned rats into three experimental groups: (1) a control group, (2) a group that received 18 h of MV and saline solution, and (3) a group that received 18 h of MV and was treated with a selective HO-1 inhibitor. Indices of oxidative stress, protease activation, and fiber atrophy were measured in the diaphragm. RESULTS: Inhibition of HO-1 activity did not prevent or exacerbate MV-induced diaphragmatic oxidative stress (as indicated by biomarkers of oxidative damage). Further, inhibition of HO-1 activity did not influence MV-induced protease activation or myofiber atrophy in the diaphragm. CONCLUSIONS: Our results indicate that HO-1 is neither a pro-oxidant nor an antioxidant in the diaphragm during MV. Furthermore, our findings reveal that HO-1 does not play an important role in MV-induced protease activation and diaphragmatic atrophy. |
| | |
Authors:
|
Darin J Falk; Andreas N Kavazis; Melissa A Whidden; Ashley J Smuder; Joseph M McClung; Matthew B Hudson; Scott K Powers |
Related Documents
:
|
20086124 - Oxidative stress and the effect of parasites on a carotenoid-based ornament. 20571914 - Protective effects of flavonoids against oxidative stress induced by simulated microgra... 15485594 - Postprandial lipid oxidation and cardiovascular disease risk. 16565714 - Elevated levels of oxidized cholesterol metabolites in lewy body disease brains acceler... 9598844 - No effect of consumption of green and black tea on plasma lipid and antioxidant levels ... 6525204 - Effect of phospholipid on aldosterone biosynthesis by a cytochrome p-450(11) beta-recon... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2010-11-24 |
Journal Detail:
|
Title: Chest Volume: 139 ISSN: 1931-3543 ISO Abbreviation: Chest Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-04-06 Completed Date: 2011-06-07 Revised Date: 2012-09-18 |
Medline Journal Info:
|
Nlm Unique ID: 0231335 Medline TA: Chest Country: United States |
Other Details:
|
Languages: eng Pagination: 816-24 Citation Subset: AIM; IM |
Affiliation:
|
Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Diaphragm / metabolism*, pathology Disease Models, Animal Female Follow-Up Studies Heme Oxygenase-1 / antagonists & inhibitors, metabolism* Muscular Atrophy / etiology, metabolism*, pathology Oxidative Stress / physiology* Rats Rats, Sprague-Dawley Respiration, Artificial / adverse effects* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HL072789/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 1.14.99.3/Heme Oxygenase-1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Longitudinal shortening accounts for the majority of right ventricular contraction and improves afte...
Next Document: Health-care-associated bloodstream infections at admission to the ICU.