Document Detail

Mechanical ventilation causes monocyte deactivation in intact and endotoxin-treated mice.
MedLine Citation:
PMID:  18301217     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Monocyte deactivation, defined as the decrease of surface expression of class II molecules of the main histocompatibility complex (MHC) on circulating monocytes, can occur after severe injuries, like trauma, sepsis, or major surgery. We hypothesized that mechanical ventilation could also be a cause. METHODS: Prospective experimental study. Intact and endotoxin-treated (20 mg/kg of intraperitoneal lipopolysaccharide, 4 hours before the experiment) Swiss mice were tracheotomized and ventilated with one of four possible ventilatory settings: control (no ventilation), low pressure (peak pressure 20 cm H2O, positive end-expiratory pressure [PEEP] 4 cm H2O), high pressure (peak pressure 30 cm H2O, PEEP 0 cm H2O), or high pressure plus an intraperitoneal dose of interferon (IFN)-gamma (40,000 units). After 1 hour, an arterial blood sample was obtained, and the right lung removed to measure gas exchange and the lung wet-to-dry weight ratio. Expression of class II MHC molecules was assessed in peripheral monocytes using flow cytometry. RESULTS: High-pressure ventilation was related to a decrease in oxygenation and to an increase in lung wet-to-dry weight ratio. The expression of class II MHC molecules in blood monocytes decreased in the high-pressure group, but not in IFN-gamma-treated mice. The results were similar in both intact and endotoxin-treated mice. CONCLUSIONS: Mechanical ventilation with high pressure and zero PEEP can cause monocyte deactivation. This phenomenon can be avoided by treatment with IFN-gamma.
Guillermo M Albaiceta; Angeles Fernandez; Diego Parra; Jose Antonio Gonzalo; Emilio García-Prieto; Francisco Taboada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of trauma     Volume:  64     ISSN:  1529-8809     ISO Abbreviation:  J Trauma     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-27     Completed Date:  2008-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376373     Medline TA:  J Trauma     Country:  United States    
Other Details:
Languages:  eng     Pagination:  470-6     Citation Subset:  AIM; IM    
Intensive Care Unit, Department of Haematology, Hospital Universitario Central de Asturias, University of Oviedo, Oviedo, Spain.
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MeSH Terms
Genes, MHC Class II / physiology*
Interferon-gamma / therapeutic use
Lung / cytology*
Monocytes / metabolism*
Positive-Pressure Respiration
Prospective Studies
Respiration, Artificial*
Reg. No./Substance:
0/Lipopolysaccharides; 82115-62-6/Interferon-gamma

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