| Mechanical stress enhances production of cytokines in human periodontal ligament cells induced by Porphyromonas gingivalis. | |
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MedLine Citation:
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PMID: 20970115 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: We have previously reported that human periodontal ligament (hPDL) cells produced many kinds of cytokines as a result of bacterial stimulation, including stimulation with Porphyromonas gingivalis (P. gingivalis). However, the effects of mechanical stress on cytokine production in hPDL cells stimulated by periodontopathogenic bacteria are not clearly understood. In this study, we investigated the effects of mechanical stress on the production of inflammatory cytokines in hPDL cells induced by stimulation with P. gingivalis. METHODS: The hPDL cells were exposed to various levels of mechanical stress (1, 6, 10 and 50MPa) and costimulated with mechanical stress and P. gingivalis for 24h. Cytokine mRNA expressions were determined by RT-PCR. Cytokines in the culture supernatant were assessed by ELISA, and morphologic changes in hPDL cells were observed. RESULTS: The expressions of interleukin (IL)-6, IL-8 and tumor necrosis factor-α mRNA were observed in hPDL cells after exposure to mechanical stress. Moreover, the production of IL-6 and IL-8 increased significantly after exposure to mechanical stress ranging from 1 to 10MPa. The amount of IL-8 in the culture supernatants of hPDL cells costimulated with P. gingivalis and mechanical stress was significantly higher than the expected additive amount. The morphology of hPDL cells did not change after exposure to 6MPa, but these cells were partly detached from the Petri dish after exposure to 50MPa. CONCLUSIONS: These results suggest that local inflammation of the periodontal ligament may be induced mainly by periodontal bacteria, and mechanical stress may promote local inflammation. |
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Authors:
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Toshiro Yamamoto; Masakazu Kita; Kenta Yamamoto; Yuki Akamatsu; Fumishige Oseko; Narisato Kanamura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of oral biology Volume: 56 ISSN: 1879-1506 ISO Abbreviation: Arch. Oral Biol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: England |
Other Details:
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Languages: eng Pagination: 251-7 Citation Subset: D; IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Dental Medicine, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, 465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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