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Mechanical stimulation of bone cells using fluid flow.
MedLine Citation:
PMID:  22130953     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
Authors:
Carmen Huesa; Astrid D Bakker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  816     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-12-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  573-92     Citation Subset:  IM    
Affiliation:
Department of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh, UK, carmen.huesa@roslin.ed.ac.uk.
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