| Mechanical stimulation of bone cells using fluid flow. | |
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MedLine Citation:
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PMID: 22130953 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described. |
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Authors:
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Carmen Huesa; Astrid D Bakker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 816 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-12-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 573-92 Citation Subset: IM |
Affiliation:
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Department of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh, UK, carmen.huesa@roslin.ed.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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